Sano Masakazu, Genkai Nobuyuki, Yajima Naoki, Tsuchiya Naoto, Homma Junpei, Tanaka Ryuichi, Miki Toru, Yamanaka Ryuya
Department of Neurosurgery, Brain Research Institute, Niigata University, Niigata 951-8585, Japan.
Oncol Rep. 2006 Nov;16(5):1093-8.
The ECT2 (epithelial cell transforming sequence 2) proto-oncogene encodes a guanine nucleotide exchange factor for Rho GTPases, and regulates cytokinesis. ECT2 plays a critical role in Rho activation during cytokinesis, and thus may play a role in the pathogenesis of glioma. In this study, we investigated relationships between ECT2 expression, tumor histology, and prognosis in glioma patients. ECT2 mRNA expression was examined using quantitative real-time PCR, while its protein expression was examined by immunohistochemistry of 54 glioma tissue samples. Expressions of ECT2 mRNA and protein were markedly increased in high-grade gliomas compared to low-grade gliomas. Patients in whom expression of ECT2 mRNA and protein in tumor tissues was the lowest survived longer than patients who had higher expression. In vitro, ECT2 siRNA inhibited glioma cell proliferation and invasion. These data suggest that increased expression of ECT2 contribute to promotion of tumor invasiveness and progression, implying that evaluation of ECT2 expression is a prognostic marker for glioma patients.
ECT2（上皮细胞转化序列2）原癌基因编码一种Rho GTP酶的鸟嘌呤核苷酸交换因子，并调节胞质分裂。ECT2在胞质分裂过程中的Rho激活中起关键作用，因此可能在胶质瘤的发病机制中发挥作用。在本研究中，我们调查了胶质瘤患者中ECT2表达、肿瘤组织学和预后之间的关系。使用定量实时PCR检测ECT2 mRNA表达，同时通过对54例胶质瘤组织样本进行免疫组织化学检测其蛋白表达。与低级别胶质瘤相比，高级别胶质瘤中ECT2 mRNA和蛋白的表达明显增加。肿瘤组织中ECT2 mRNA和蛋白表达最低的患者比表达较高的患者存活时间更长。在体外，ECT2 siRNA抑制胶质瘤细胞的增殖和侵袭。这些数据表明，ECT2表达增加有助于促进肿瘤侵袭和进展，这意味着评估ECT2表达是胶质瘤患者的预后标志物。
