广州管圆线虫成虫单克隆抗体的特性分析

Characterization of monoclonal antibodies to young-adult worms of Angiostrongylus cantonensis.

作者信息

Chang J H, Yen C M, Chen E R

机构信息

Department of Parasitology, National Cheng Kung University Medical College, Tainan, Taiwan, ROC.

出版信息

Hybridoma. 1990 Oct;9(5):465-71. doi: 10.1089/hyb.1990.9.465.

DOI:10.1089/hyb.1990.9.465

PMID:1701759

Abstract

Three IgG1 and one IgG2a monoclonal antibodies were obtained by fusion of NS-1 cells with spleen cells of BALB/C mice immunized with young-adult worm antigen of A. cantonensis. These 4 monoclonal antibodies were specific to A. cantonensis without cross-reactivity to any antigens of Toxocara canis, Ascaris suum, Clonorchis sinensis, Dirofilaria immitis, Paragonimus westermani, Gnathostoma spinigerum, Strongyloides stercoralis and Anisakis spp. detected by ELISA. The affinity constants of monoclonal antibodies specific to young-adult worms antigen of A. cantonensis were in the range of 10(7) M-1 to 10(9) M-1. The immunoblots showed that the 4 monoclonal antibodies recognized epitope on molecular weight of 204 kiloDaltons. They will be utilized to purify their corresponding antigen by an immunoaffinity column for the specific immunodiagnosis.

摘要

将NS-1细胞与用广州管圆线虫成虫抗原免疫的BALB/C小鼠的脾细胞融合，获得了三种IgG1和一种IgG2a单克隆抗体。通过ELISA检测，这4种单克隆抗体对广州管圆线虫具有特异性，与犬弓首蛔虫、猪蛔虫、华支睾吸虫、犬恶丝虫、卫氏并殖吸虫、棘颚口线虫、粪类圆线虫和异尖线虫属的任何抗原均无交叉反应。针对广州管圆线虫成虫抗原的单克隆抗体的亲和常数在10(7) M-1至10(9) M-1范围内。免疫印迹显示，这4种单克隆抗体识别分子量为204千道尔顿的表位。它们将用于通过免疫亲和柱纯化其相应抗原，用于特异性免疫诊断。