Spertini F, Stohl W, Ramesh N, Moody C, Geha R S
Division of Immunology, Children's Hospital, Boston, MA 02115.
J Immunol. 1991 Jan 1;146(1):47-52.
A mouse mAb, TS 43, which recognized the human CD5 molecule, was found to induce the proliferation of human peripheral blood T cells. TS 43 mAb precipitated from 125I-radiolabeled T cells a 67-kDa band, which comigrated with the 67-kDa band precipitated by the anti-CD5 mAb OKT1. Preclearing of cell lysates with OKT1 mAb abolished the capacity of TS 43 mAb to precipitate radiolabeled material from T cell lysates. Furthermore, a mouse T cell hybridoma transfected with human CD5 was stained by TS 43 mAb. T cell proliferation mediated by TS 43 mAb was monocyte dependent, and was accompanied by IL-2R expression and by IL-2 synthesis. T cell activation by TS 43 mAb involved a rise in intracellular calcium level (CA2+)i and was dependent on the expression of the TCR/CD3 complex because no rise in (Ca2+)i was observed in a TCR-beta-deficient Jurkat T cell mutant. This study indicates that CD5 should be added to the list of surface molecules that can signal T cells to proliferate.
一种识别人类CD5分子的小鼠单克隆抗体TS 43，被发现可诱导人外周血T细胞增殖。TS 43单克隆抗体从125I放射性标记的T细胞中沉淀出一条67 kDa的条带，该条带与抗CD5单克隆抗体OKT1沉淀出的67 kDa条带迁移率相同。用OKT1单克隆抗体预先清除细胞裂解物后，TS 43单克隆抗体从T细胞裂解物中沉淀放射性标记物质的能力消失。此外，转染了人类CD5的小鼠T细胞杂交瘤可被TS 43单克隆抗体染色。TS 43单克隆抗体介导的T细胞增殖依赖于单核细胞，并伴有IL-2R表达和IL-2合成。TS 43单克隆抗体激活T细胞涉及细胞内钙水平（Ca2+）i升高，且依赖于TCR/CD3复合物的表达，因为在TCR-β缺陷的Jurkat T细胞突变体中未观察到（Ca2+）i升高。这项研究表明，CD5应被添加到能够向T细胞发出增殖信号的表面分子列表中。
