Levine S, Saltzman A, Deibler G E
Pathology Department, New York Medical College, Valhalla 10595.
Proc Soc Exp Biol Med. 1990 Dec;195(3):324-8.
Immunization with peptides is usually done with the aid of Freund's adjuvant. Using peptides derived from myelin basic protein, we show that aqueous solutions can be antigenic (encephalitogenic in this instance) in Lewis rats. The first procedure involved multiple doses of aqueous peptide, increased absorption into the lymphatic system from the peritoneal cavity in the postinflammatory state, and the use of pertussis vaccine. Three different peptides containing the major encephalitogenic site were active in this system, with the activity somewhat proportional to the size of the fragment. The second procedure, the direct delivery of peptide to lymph nodes by percutaneous inoculation, was equally successful and did not require the use of pertussis vaccine.
用肽进行免疫通常借助弗氏佐剂来完成。利用源自髓鞘碱性蛋白的肽，我们发现水溶液在Lewis大鼠中可具有抗原性（在这种情况下具有致脑炎作用）。第一种方法包括多次给予水性肽、在炎症后状态下增加从腹腔向淋巴系统的吸收以及使用百日咳疫苗。三种含有主要致脑炎位点的不同肽在该系统中具有活性，其活性与片段大小有些成比例。第二种方法，即通过经皮接种将肽直接递送至淋巴结，同样成功且无需使用百日咳疫苗。
