Levine S, Saltzman A, Deibler G E
Pathology Department, New York Medical College, Valhalla 10595.
J Neuropathol Exp Neurol. 1990 Sep;49(5):480-5. doi: 10.1097/00005072-199009000-00003.
The induction of experimental allergic encephalomyelitis (EAE) with purified myelin basic protein (MBP) has, heretofore, required its incorporation in a water-in-oil emulsion or adsorption on particulate adjuvants. In the present work, the absorption of a saline solution of MBP from the peritoneal cavity into the mediastinal lymph nodes was increased by giving repeated inoculations or by pretreating rats with a peritoneal irritant. Under these conditions, the only adjuvant needed for production of EAE was aqueous pertussis vaccine which was injected separately a few hours or one day after the MBP. Pertussis vaccine was also necessary for production of EAE with intradermal injection of aqueous MBP. By injecting the aqueous MBP directly into pre-enlarged popliteal lymph nodes, it was possible to produce EAE without the pertussis vaccine. Thus, EAE can be induced in rats using MBP without the addition of Freund's adjuvant or pertussis vaccine.
迄今为止，用纯化的髓鞘碱性蛋白（MBP）诱导实验性变应性脑脊髓炎（EAE），需要将其掺入油包水乳液中或吸附在颗粒佐剂上。在本研究中，通过重复接种或用腹膜刺激剂预处理大鼠，可增加腹膜腔内MBP盐溶液向纵隔淋巴结的吸收。在这些条件下，产生EAE所需的唯一佐剂是百日咳疫苗水剂，它在MBP注射后数小时或一天单独注射。用MBP盐水溶液皮内注射产生EAE也需要百日咳疫苗。通过将MBP盐水溶液直接注入预先肿大的腘淋巴结，不使用百日咳疫苗也有可能产生EAE。因此，使用MBP在大鼠中可诱导EAE，而无需添加弗氏佐剂或百日咳疫苗。
