Misharin Alexander S, Zubarev Roman A
Laboratory for Biological and Medical Mass Spectrometry, Uppsala University, Box 583, Uppsala S-751 23, Sweden.
Rapid Commun Mass Spectrom. 2006;20(21):3223-8. doi: 10.1002/rcm.2724.
Separation of the functions of ion excitation and detection between different cell compartments allows for implementation of excitation and detection techniques unattainable in a single compartment of the conventional ion cyclotron resonance (ICR) cell. In particular, multi-electrode detection at a multiple of the main cyclotron frequency can be utilized without the loss of sensitivity and other negative effects. The new O-trap designed exclusively for ion detection adds an additional, internal coaxial cylinder around which ions with excited cyclotron orbits rotate. Comparison of simulated performance characteristics of the new O-trap with those of the same-size conventional cylindrical cell shows that the O-trap can provide higher sensitivity and ion capacity. Multiplexing of the O-traps can further increase the analysis speed. Future efforts will be aimed at building and testing experimentally the coaxial O-trap, including optimization of the method of ion transfer between the compartments of the cell.
在不同细胞区室之间分离离子激发和检测功能，使得在传统离子回旋共振（ICR）细胞的单个区室中无法实现的激发和检测技术得以实施。特别是，可以利用在主回旋频率倍数下的多电极检测，而不会损失灵敏度和产生其他负面影响。专门为离子检测设计的新型O形阱增加了一个额外的内部同轴圆柱，具有激发回旋轨道的离子围绕该圆柱旋转。将新型O形阱的模拟性能特征与相同尺寸的传统圆柱形细胞的性能特征进行比较表明，O形阱可以提供更高的灵敏度和离子容量。O形阱的多路复用可以进一步提高分析速度。未来的工作将致力于构建并通过实验测试同轴O形阱，包括优化细胞区室之间的离子转移方法。
