Broekaert D, Goeman L, Ramaekers F C, Van Muijen G N, Eto H, Lane E B, Leigh I M, De Bersaques J, Coucke P
Laboratory of Physiological Chemistry, Faculty of Medicine, State University of Ghent, Belgium.
Arch Dermatol Res. 1990;282(6):383-91. doi: 10.1007/BF00372089.
The differentiation state of skin epithelial cysts and some uncommon types of epithelial skin tumours was investigated by immunohistochemical staining, mainly using cytokeratin (CK) polypeptide-specific monoclonal antibodies. Samples of interfollicular epidermis, hair follicles and eccrine sweat glands were included as reference tissues. The CK reactivity in epidermoid cysts and milia is not restricted to CKs involved in epidermal-type differentiation, i.e. CK1, 5, 10 and 14, but in addition CK16, a hyperproliferative keratinocyte marker is suprabasally expressed. CK1 and 10 are other prominent suprabasal markers, while CK14 seems to be preferentially expressed in the basal cell layer. Of the non-epidermal CKs, only CK4 was focally or more extensively detected in about 50% of the cases. In terms of CK reactivity, keratinization of trichilemmal cysts corresponds to the keratinization of the anagen-phase hair follicle in the isthmus. The CK reactivity is again restricted to CK1, 5, 10, 14 and 16. However, the CK1 as well as CK10 reactivity is subject to serious limitations, since both CKs were only convincingly observed in foci of terminal differentiation. Eccrine hydrocystoma obligatorily expresses a complex CK set, including CK7, 8, 14, 18 and 19. This CK set perfectly corresponds to the CK composition observed in acini of eccrine sweat glands. In addition, a discontinuous CK4 and 16 reactivity was seen in about 50% of the sites, while CK1 and 10 displayed a strictly focal appearance. On the other hand, syringoma produces in its distinct structures, a CK pattern reminiscent of the one observed in eccrine sweat gland ducts and includes CK1, 5, 10, 14, 16 and 19.(ABSTRACT TRUNCATED AT 250 WORDS)
通过免疫组织化学染色，主要使用细胞角蛋白（CK）多肽特异性单克隆抗体，研究了皮肤上皮囊肿和一些不常见类型的上皮性皮肤肿瘤的分化状态。包括毛囊间表皮、毛囊和小汗腺的样本作为对照组织。表皮样囊肿和粟丘疹中的CK反应不仅限于参与表皮型分化的CK，即CK1、5、10和14，此外，作为增殖性角质形成细胞标志物的CK16在基底上层表达。CK1和10是其他突出的基底上层标志物，而CK14似乎优先在基底细胞层表达。在非表皮CK中，仅约50%的病例中局部或更广泛地检测到CK4。就CK反应而言，毛鞘囊肿的角化对应于峡部生长期毛囊的角化。CK反应再次限于CK1、5、10、14和16。然而，CK1以及CK10的反应受到严重限制，因为仅在终末分化灶中令人信服地观察到这两种CK。小汗腺汗囊肿必然表达一组复杂的CK，包括CK7、8、14、18和19。这组CK与在小汗腺腺泡中观察到的CK组成完全对应。此外，约50%的部位可见不连续的CK4和16反应，而CK1和10呈严格的局灶性表现。另一方面，汗管瘤在其独特结构中产生一种CK模式，让人联想到在小汗腺导管中观察到的模式，包括CK1、5、10、14、16和19。（摘要截短于250字）
