Eckert F, Nilles M, Schmid U, Altmannsberger M
Department of Dermatology, University of Munich, Germany.
Am J Dermatopathol. 1992 Apr;14(2):115-21. doi: 10.1097/00000372-199204000-00005.
The distribution of cytokeratin (CK) polypeptides expressed in syringomas (12 cases) was compared with that in normal eccrine sweat ducts using immunohistochemical techniques on paraffin-embedded tissue. Intradermal and intraepidermal segments of the eccrine duct showed reactivity with an antibody to CK1/5/10/11 in all cell layers, whereas CK19 expression was restricted to the luminal cell layer. CK14 was expressed in all cells of the eccrine duct except for the peripheral cells of the intraepidermal duct. Expression of CK5/6 was seen in the basal cells of the dermal duct and of the lower intraepidermal duct (sweat duct ridge) exclusively. Reactivity with an antibody to CK1 was found in the intermediate cells of the uppermost part of the eccrine dermal duct. In addition, this antibody gave a strong staining of the peripheral cells of the intraepidermal duct, leaving basal cells of the sweat duct ridge and luminal cells unstained. In syringoma, CK distribution was essentially comparable with that found in the uppermost part of the dermal duct and in the sweat duct ridge. Namely, ductal luminal cells expressed CK1/5/10/11, CK19, and variably CK14. Intermediate cells of ductal structures and solid nests were homogeneously stained by antibodies to CK1 and CK1/5/10/11, whereas CK14 was expressed heterogeneously. The basal or outermost layer of ductal structures and solid nests was reactive with antibodies to CK1/5/10/11, CK5/6, and CK14. With regard to CK expression, the results indicate that syringoma represents a tumor differentiating toward both the uppermost part of the dermal duct and the lower intraepidermal duct (sweat duct ridge) of the eccrine sweat gland.
运用免疫组织化学技术，对石蜡包埋组织中汗管瘤（12例）和正常小汗腺导管中细胞角蛋白（CK）多肽的分布进行了比较。小汗腺导管的真皮内段和表皮内段在所有细胞层均显示出与抗CK1/5/10/11抗体的反应性，而CK19的表达局限于管腔细胞层。CK14在小汗腺导管的所有细胞中均有表达，但表皮内导管的外周细胞除外。CK5/6仅在真皮导管和表皮内导管下部（汗腺导管嵴）的基底细胞中表达。在小汗腺真皮导管最上部的中间细胞中发现了与抗CK1抗体的反应性。此外，该抗体对表皮内导管的外周细胞有强烈染色，而汗腺导管嵴的基底细胞和管腔细胞未被染色。在汗管瘤中，CK分布与在真皮导管最上部和汗腺导管嵴中发现的情况基本相当。也就是说，导管管腔细胞表达CK1/5/10/11、CK19，且CK14表达情况不一。导管结构和实性巢的中间细胞被抗CK1和抗CK1/5/10/1l抗体均匀染色，而CK14表达不均一。导管结构和实性巢的基底或最外层与抗CK1/5/10/11、抗CK5/6和抗CK14抗体发生反应。关于CK表达，结果表明汗管瘤是一种向小汗腺真皮导管最上部和表皮内导管下部（汗腺导管嵴）分化的肿瘤。
