Ayiguli Tashi, Zhou Qun, Dong Xiao-Ou, Sun Su-Qin
Department of Chemistry, Tsinghua University, Beijing 100084, China.
Guang Pu Xue Yu Guang Pu Fen Xi. 2006 Jul;26(7):1238-41.
Standard Gancao (Glycyrrhiza uralensis Fisch) and false Ciguogancao (Glycyrrhiza pallidiflata Batal) were identified fast, nondestructively by Fourier transform infrared spectroscopy (FTIR) combined with derivative spectra and two-dimensional correlation spectroscopy (2D) in the present article. The result shows that although the two kinds of Gancao belong to one genus, there are some certain differences in their chemical components that are reflected in the IR spectra, but with some similarity and dissimilarity in the IR spectra. The two kinds of Gancao are quite different from each other in second derivative spectra and 2D spectra. Based on the differences reflected in the IR and 2D IR, the standard gancao can be identified from the false easily and clearly. The result also proved that there is a relationship between the IR spectra and the chemical components of the herbs. This method is fast, accurate and nondestructive, and the wastage of sample is less. The fast, accurate property of 2D spectroscopy makes it a powerful and new approach to evaluating medicinal herbs impersonally.
本文采用傅里叶变换红外光谱（FTIR）结合导数光谱和二维相关光谱（2D），对正品甘草（乌拉尔甘草）和伪品刺果甘草进行了快速、无损鉴别。结果表明，虽然两种甘草同属一个属，但它们的化学成分在红外光谱中存在一定差异，红外光谱既有相似性也有差异性。两种甘草在二阶导数光谱和二维光谱上有很大不同。基于红外光谱和二维红外光谱所反映的差异，可以轻松、清晰地将正品甘草与伪品区分开来。结果还证明了红外光谱与药材化学成分之间存在关联。该方法快速、准确、无损，且样品损耗少。二维光谱的快速、准确特性使其成为客观评价药材的一种有力新方法。
