Uchida Ryuichi, Pandey Basu Dev, Sherchand Jeevan Bahadur, Ahmed Kamurddin, Yokoo Michiyo, Nakagomi Toyoko, Cuevas Luis E, Cunliffe Nigel A, Hart C A, Nakagomi Osamu
Division of Molecular Epidemiology, Graduate School of Biomedical Sciences, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan.
J Clin Microbiol. 2006 Oct;44(10):3499-505. doi: 10.1128/JCM.01089-06.
In anticipation of a rotavirus vaccine in Nepal, this study was undertaken to determine the distribution of the G and P serotypes and electropherotypes of rotaviruses in order to examine if there is any emerging serotype or unusual strain circulating in children and adults in Nepal. Of 1,315 diarrheal stool specimens, rotavirus was detected by an enzyme-linked immunosorbent assay in 116 (17%) of 666 patients less than 5 years of age, in 18 (7%) of 260 patients 5 to 14 years of age, and in 19 (5%) of 358 patients 15 years of age and older. Approximately 75% of rotavirus diarrhea occurred in children less than 5 years of age. Approximately 70% of rotaviruses found in each of the three age groups belonged to serotype G1P[8]. Interestingly, there were 29 (20%) G12 rotaviruses carrying either P[8] or P[6] and one (0.7%) G11 rotavirus carrying an unusual P[25] genotype. RNA polyacrylamide gel electrophoresis discriminated 19 strains (electropherotypes), among which there were three codominant strains carrying G1P[8] and long RNA patterns. Five electropherotypes were discriminated among G12 rotaviruses, all of which had long RNA patterns. The fact that 20% of rotaviruses were G12 strains carrying either P[8] or P[6] and had multiple electropherotypes suggest that G12 strains are not more rare strains but that they pose an emerging challenge to current and future vaccines. The presence of multiple strains as defined by electropherotypes suggests the richness of the rotavirus gene pool in Nepal, where unusual strains may continue to emerge.
鉴于尼泊尔即将引入轮状病毒疫苗，开展了本研究以确定轮状病毒的G和P血清型及电泳型分布，从而检测尼泊尔儿童和成人中是否有任何新出现的血清型或异常毒株在传播。在1315份腹泻粪便标本中，通过酶联免疫吸附试验在666名5岁以下患者中的116例（17%）、260名5至14岁患者中的18例（7%）以及358名15岁及以上患者中的19例（5%）检测到轮状病毒。约75%的轮状病毒腹泻发生在5岁以下儿童中。在三个年龄组中发现的轮状病毒约70%属于G1P[8]血清型。有趣的是，有29株（20%）G12轮状病毒携带P[8]或P[6]，还有1株（0.7%）G11轮状病毒携带异常的P[25]基因型。RNA聚丙烯酰胺凝胶电泳区分出19个毒株（电泳型），其中有3个共显性毒株携带G1P[8]和长RNA模式。在G12轮状病毒中区分出5种电泳型，它们均具有长RNA模式。20%的轮状病毒为携带P[8]或P[6]的G12毒株且具有多种电泳型，这一事实表明G12毒株并非更罕见的毒株，而是对当前和未来的疫苗构成了新出现的挑战。由电泳型定义的多种毒株的存在表明尼泊尔轮状病毒基因库丰富，异常毒株可能会继续出现。
