el Ridi R, Bubeník H
Int J Cancer. 1975 Jul 15;16(1):83-90. doi: 10.1002/ijc.2910160110.
A factor inhibiting tumour growth in syngeneic hosts was found in the sera of inbred Lewis rats carrying Rous sarcoma virus-induced tumour (RSL). The findings presented here suggest that the serum factor is a tumour-associated transplantation antigen (TATA) shed from the neoplasm into the circulation. All the tumour bearers' sera tested with RSL cells were negative in indirect membrane immunofluorescence;however, on passive transfer into syngeneic rats, they protected the animals against the growth of an RSL tumour inoculum. A similar protective effect was also observed after injection of TATA prepared from RSL cell membranes by solubilization with potassium cholate. When incorporated into Freund's adjuvant, tumour-bearers' sera immunized the animals against a subsequent RSL sarcoma graft. Sera collected from immunosuppressed rats bearing large sarcomas which presumably contain neither tumour-specific antibody nor antigen-antibody complexes, transferred inhibition of tumour growth to syngeneic hosts. Intact immunological reactivity of recipients was a necessary prerequisite for the protective effect of sera, since the passive transfer of an inhibitory serum to immunosuppressed rats did not inhibit tumour growth. We assume that the TATA present in tumour-bearers' serum is released from the growing neoplasm as a result of either cell death or membrane metabolic turnover.
在携带劳氏肉瘤病毒诱导肿瘤（RSL）的近交系Lewis大鼠血清中发现了一种抑制同基因宿主肿瘤生长的因子。此处呈现的研究结果表明，血清因子是一种肿瘤相关移植抗原（TATA），它从肿瘤中释放到循环系统中。用RSL细胞检测所有肿瘤携带者的血清，间接膜免疫荧光结果均为阴性；然而，将这些血清被动转移到同基因大鼠体内后，它们能保护动物免受RSL肿瘤接种物的生长影响。在用胆酸钾溶解从RSL细胞膜制备的TATA注射后，也观察到了类似的保护作用。当将肿瘤携带者的血清掺入弗氏佐剂中时，能使动物对随后移植的RSL肉瘤产生免疫。从患有大肉瘤的免疫抑制大鼠中收集的血清，这些大鼠可能既不含有肿瘤特异性抗体也不含有抗原 - 抗体复合物，能将肿瘤生长抑制作用转移给同基因宿主。受体完整的免疫反应性是血清发挥保护作用的必要前提，因为将抑制性血清被动转移到免疫抑制大鼠体内并不能抑制肿瘤生长。我们推测，肿瘤携带者血清中存在的TATA是由于细胞死亡或膜代谢更新从生长中的肿瘤释放出来的。
