Inui T, Ochi Y, Morishima M
Central Clinical Laboratory, Shiga University of Medical Science, Otsu.
Rinsho Byori. 1990 Oct;38(10):1141-8.
Anti-idiotypic (anti-ID) antibody in test serum was determined by the direct binding assay using 125I-anti-human thyroglobulin (hTg). Several positive cases were found in Graves' disease and thyroiditis chronica. Positive anti-ID antibodies could be classified into two types. Type 1 showed the positive anti-hTg antibody and high Tg levels by RIA using double antibody method. Type 2 showed the positive anti-hTg antibody but low Tg levels by RIA. The binding of 125I-hTg to anti-hTg antibody was displaced by anti-ID antibody in type 1, but was not anti-ID antibody in type 2. A case of coexistence of autoantibody to hTSH and auto-anti-ID antibody to anti-hTSH antibody was found. She showed normal thyroid function (T4, T3), but TSH level showed discrepancy by different assay methods. Both autoantibodies for hTSH and for anti-hTSH antibody were demonstrated by the reaction of patient's antibody with both 125I-hTSH and 125I-anti-hTSH (MoAb). These two autoantibodies belong to the polyclonal IgG. The autoantibody for hTSH recognized only the beta-subunit of hTSH. Neither stimulating type of TSH receptor antibody (TRAb) nor blocking type of TRAb interfered with the binding of patient's anti-ID to 125I-anti-hTSH. This binding reaction could be inhibited by the unlabeled hTSH. This anti-ID might represent the internal image of the non-biological active site of TSH molecule, because of absence of thyroid stimulating activity. These anti-ID antibodies may provide evidence supporting a network theory of the immune system.
采用¹²⁵I-抗人甲状腺球蛋白（hTg）直接结合试验测定检测血清中的抗独特型（抗-ID）抗体。在格雷夫斯病和慢性甲状腺炎中发现了几例阳性病例。阳性抗-ID抗体可分为两种类型。1型通过双抗体法放射免疫分析显示抗-hTg抗体阳性且Tg水平高。2型通过放射免疫分析显示抗-hTg抗体阳性但Tg水平低。在1型中，¹²⁵I-hTg与抗-hTg抗体的结合被抗-ID抗体取代，但在2型中则未被抗-ID抗体取代。发现1例同时存在抗hTSH自身抗体和抗抗-hTSH抗体的自身抗-ID抗体。她的甲状腺功能（T4、T3）正常，但不同检测方法显示TSH水平存在差异。患者抗体与¹²⁵I-hTSH和¹²⁵I-抗-hTSH（单克隆抗体）的反应均证实了抗hTSH和抗抗-hTSH抗体这两种自身抗体。这两种自身抗体属于多克隆IgG。抗hTSH自身抗体仅识别hTSH的β亚基。刺激型促甲状腺激素受体抗体（TRAb）和阻断型TRAb均不干扰患者抗-ID与¹²⁵I-抗-hTSH的结合。这种结合反应可被未标记的hTSH抑制。由于缺乏甲状腺刺激活性，这种抗-ID可能代表TSH分子非生物活性位点的内影像。这些抗-ID抗体可能为免疫系统的网络理论提供证据支持。
