Lin Chi-Wen, Tsai Shen-Long, Hou Sung-Nan
Department of Environmental Engineering, Da-Yeh University, 112 Shan-Jiau Rd. Da-Tsuen, Changhua, 515, Taiwan, Republic of China.
Appl Microbiol Biotechnol. 2007 Feb;74(1):194-201. doi: 10.1007/s00253-006-0601-5. Epub 2006 Oct 5.
A mixed culture was utilized to evaluate methyl tert-butyl ether (MTBE) removal under various conditions and to isolate a MTBE-degrading pure culture. The results showed that high MTBE removal efficiencies can be reached even in the presence of other substrates. The biodegradation sequence of the target compounds by the mixed culture, in order of removal rate, was toluene, ethyl benzene, p-xylene, benzene, MTBE, ethyl ether, tert-amyl methyl ether, and ethyl tert-butyl ether. In addition, preincubation of the mixed cultures with benzene and toluene showed no negative effect on MTBE removal; on the contrary, it could even increase the degradation rate of MTBE. The kinetic behavior showed that the maximum specific growth rate and the saturation constant of the mixed culture degrading MTBE are 0.000778 h(-1) and 0.029 mg l(-1), respectively. However, a high MTBE concentration (60 mg l(-1)) was slightly inhibiting to the growth of the mixed culture. The pure culture isolated from the enrichments in the bubble-air bioreactor showed better efficiency in MTBE removal than the mixed culture; whereas, tert-butyl alcohol was formed as a metabolic intermediate during the breakdown of MTBE.
利用混合培养物评估了在各种条件下甲基叔丁基醚（MTBE）的去除情况，并分离出一种降解MTBE的纯培养物。结果表明，即使存在其他底物，MTBE的去除效率也能达到很高。混合培养物对目标化合物的生物降解顺序，按去除率排序为甲苯、乙苯、对二甲苯、苯、MTBE、乙醚、叔戊基甲基醚和乙基叔丁基醚。此外，混合培养物与苯和甲苯的预培养对MTBE的去除没有负面影响；相反，甚至可以提高MTBE的降解率。动力学行为表明，混合培养物降解MTBE的最大比生长速率和饱和常数分别为0.000778 h(-1)和0.029 mg l(-1)。然而，高浓度的MTBE（60 mg l(-1)）对混合培养物的生长有轻微抑制作用。从鼓泡空气生物反应器中的富集物中分离出的纯培养物在MTBE去除方面比混合培养物表现出更好的效率；而在MTBE分解过程中，叔丁醇作为代谢中间产物形成。
