Yu Lei, Domann Frederick E
Free Radical and Radiation Program, Radiation Oncology Department, B180 ML, The University of Iowa, Iowa City, Iowa 52242, USA.
Biotechnol Prog. 2006 Sep-Oct;22(5):1461-3. doi: 10.1021/bp060102e.
This Note describes a novel rapid and direct quantitative method for examining the activity of genetic response elements. This method will provide an alternative to the classically used "reporter gene" activity assays. We show that a transfected genetic cis-regulatory element that responds to the transcription factor p53 gives a quantitative read-out at the RNA level that parallels that of an endogenous p53 responsive gene, p21 waf1/cip1. The correlation between the endogenous p21 gene expression in response to p53 and the transfected cis element is remarkable. This method is more direct and potentially faster than traditional promoter-reporter assays.
本笔记描述了一种用于检测遗传反应元件活性的新型快速直接定量方法。该方法将为经典使用的“报告基因”活性测定提供一种替代方法。我们表明，对转录因子p53作出反应的转染遗传顺式调控元件在RNA水平上给出的定量读数与内源性p53反应基因p21 waf1/cip1的读数相似。内源性p21基因对p53反应的表达与转染的顺式元件之间的相关性非常显著。该方法比传统的启动子-报告基因测定更直接，可能也更快。
