表面增强激光解吸/电离飞行时间（SELDI-TOF）蛋白质组学血液分析中主要血清蛋白去除的研究意义。
Interest of major serum protein removal for Surface-Enhanced Laser Desorption/Ionization - Time Of Flight (SELDI-TOF) proteomic blood profiling.
作者信息
Roche Stéphane, Tiers Laurent, Provansal Monique, Piva Marie-Thérèse, Lehmann Sylvain
机构信息
Institut de Génétique Humaine du CNRS, 141 rue de la Cardonille, 34396 Montpellier, France.
出版信息
Proteome Sci. 2006 Oct 5;4:20. doi: 10.1186/1477-5956-4-20.
Abstract
BACKGROUND
Surface-Enhanced Laser Desorption/Ionization - Time Of Flight (SELDI-TOF) has been proposed as new approach for blood biomarker discovery. However, results obtained so far have been often disappointing as this technique still has difficulties to detect low-abundant plasma and serum proteins.
RESULTS
We used a serum depletion scheme using chicken antibodies against various abundant proteins to realized a pre-fractionation of serum prior to SELDI-TOF profiling. Depletion of major serum proteins by immunocapture was confirmed by 1D and 2D gel electrophoresis. SELDI-TOF analysis of bound and unbound (depleted) serum fractions revealed that this approach allows the detection of new low abundant protein peaks with satisfactory reproducibility.
CONCLUSION
The combination of immunocapture and SELDI-TOF analysis opens new avenues into proteomic profiling for the discovery of blood biomarkers.
摘要
背景
表面增强激光解吸/电离飞行时间质谱（SELDI-TOF）已被提议作为发现血液生物标志物的新方法。然而，迄今为止获得的结果往往令人失望，因为该技术在检测低丰度血浆和血清蛋白方面仍存在困难。
结果
我们使用了一种血清去除方案，利用针对各种丰富蛋白质的鸡抗体在SELDI-TOF分析之前对血清进行预分级分离。通过一维和二维凝胶电泳证实了通过免疫捕获去除主要血清蛋白。对结合和未结合（去除）血清组分的SELDI-TOF分析表明，这种方法能够检测到新的低丰度蛋白峰，且具有令人满意的重现性。
结论
免疫捕获和SELDI-TOF分析的结合为发现血液生物标志物的蛋白质组分析开辟了新途径。
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