Falini B, Pileri S A, Flenghi L, Liberati M, Stein H, Gerli R, Minelli O, Martelli M F, Lauria F, Poggi S
Institute of Internal Medicine, University of Perugia, Italy.
Br J Haematol. 1990 Dec;76(4):460-8. doi: 10.1111/j.1365-2141.1990.tb07901.x.
A panel of monoclonal antibodies (mAbs) directed against B-cell and hairy cell leukaemia (HCL)-associated antigens was used to identify residual hairy cells in the peripheral blood and/or bone marrow samples from 20 patients with HCL, following treatment with interferon-alpha (IFN-alpha) or interferon-beta (IFN-beta). In all cases, hairy cells retained their characteristic phenotype, e.g. positivity for CD22, CD11c, CD25, CD32, and the HCL-associated trimeric protein (t-GP) recognized by the mAbs HML-1, B-ly7, LF61 and Ber-Act8. The most specific marker for identifying a small percentage of hairy cells in peripheral blood cytospins, was t-GP. In alkaline phosphatase/anti alkaline phosphatase (APAAP) stained preparations, t-GP+ hairy cells (provided with large cytoplasm and hairy surface) could be usually distinguished from t-GP+ normal lymphocytes (small-sized cells with smooth surface). In doubtful cases the percentage of residual hairy cells could exactly be estimated by double immunofluorescence staining for CD22 (B-cell marker) and t-GP. The rationale of the test is based on the finding that the small percentage (about 1%) of t-GP+ lymphocytes circulating in the peripheral blood of normal individuals are T-cells of the CD8 subset and not B-cells. The best markers for identifying residual hairy cells in routine bone marrow biopsies were CD45RA (mAb 4KB5) and CD20 (mAb L26). Immunohistological labelling was superior to morphological examination in picking up scattered hairy cells in bone marrow biopsies showing either severe hypoplasia or exuberant hyperplasia of normal haemopoietic series.
一组针对B细胞和毛细胞白血病（HCL）相关抗原的单克隆抗体（mAb）被用于识别20例HCL患者在接受α干扰素（IFN-α）或β干扰素（IFN-β）治疗后外周血和/或骨髓样本中的残留毛细胞。在所有病例中，毛细胞保留了其特征性表型，例如对CD22、CD11c、CD25、CD32呈阳性，以及对单克隆抗体HML-1、B-ly7、LF61和Ber-Act8识别的HCL相关三聚体蛋白（t-GP）呈阳性。在外周血细胞涂片上识别少量毛细胞的最特异性标志物是t-GP。在碱性磷酸酶/抗碱性磷酸酶（APAAP）染色的制剂中，t-GP阳性的毛细胞（具有大的细胞质和毛状表面）通常可以与t-GP阳性的正常淋巴细胞（表面光滑的小细胞）区分开来。在可疑病例中，通过对CD22（B细胞标志物）和t-GP进行双重免疫荧光染色可以准确估计残留毛细胞的百分比。该检测的原理基于以下发现：正常个体外周血中循环的少量（约1%）t-GP阳性淋巴细胞是CD8亚群的T细胞而非B细胞。在常规骨髓活检中识别残留毛细胞的最佳标志物是CD45RA（单克隆抗体4KB5）和CD20（单克隆抗体L26）。在显示正常造血系列严重发育不全或过度增生的骨髓活检中，免疫组织学标记在检测散在的毛细胞方面优于形态学检查。
