Zav'yalov V P, Denesyuk A I, Zav'yalova G A
Institute of Immunology, Moscow Region, Chekhov District, U.S.S.R.
Biochim Biophys Acta. 1990 Nov 15;1041(2):178-85. doi: 10.1016/0167-4838(90)90063-l.
According to the results of the theoretical conformation analysis and available experimental data, the known immunocytokines can be divided into two groups: alpha-helical (IFNs-alpha, beta, gamma, omega; IL-2, 3, 4, 5, 6, 7; G-, M-, GM-CSFs; cMGF, PDGF) and beta-pleated proteins (ILs-1 alpha, beta; TNFS-alpha, beta). IFNs-alpha, beta, gamma, omega, IL-6, G-CSF, cMGF were shown to form a family of alpha-helical globular proteins characterized by a statistically significant homology in amino acid sequences and by common features of the secondary structure formation. Comparison of the sequences of 72 IFNs-alpha, beta, omega reveals three clusters of conservative amino acid positions. Their participation in the formation of active sites of IFN-alpha, beta, omega is supposed.
根据理论构象分析结果和现有实验数据，已知的免疫细胞因子可分为两组：α-螺旋（α、β、γ、ω干扰素；白细胞介素-2、3、4、5、6、7；粒细胞集落刺激因子、巨噬细胞集落刺激因子、粒细胞巨噬细胞集落刺激因子；心肌生长因子、血小板衍生生长因子）和β-折叠蛋白（白细胞介素-1α、β；肿瘤坏死因子-α、β）。已证明α、β、γ、ω干扰素、白细胞介素-6、粒细胞集落刺激因子、心肌生长因子形成一个α-螺旋球状蛋白家族，其特征在于氨基酸序列具有统计学上显著的同源性以及二级结构形成的共同特征。对72种α、β、ω干扰素序列的比较揭示了三个保守氨基酸位置簇。推测它们参与了α、β、ω干扰素活性位点的形成。
