关于补体第一成分的一个亚成分C1q与掺入人工膜的明尼苏达沙门氏菌孔蛋白相互作用的研究。

Studies on the interaction of C1q, a subcomponent of the first component of complement, with porins from Salmonella minnesota incorporated into artificial membranes.

作者信息

Latsch M, Möllerfeld J, Ringsdorf H, Loos M

机构信息

Institute of Medical Microbiology, Johannes Gutenberg-University, Mainz, FRG.

出版信息

FEBS Lett. 1990 Dec 10;276(1-2):201-4. doi: 10.1016/0014-5793(90)80542-q.

DOI:10.1016/0014-5793(90)80542-q

PMID:1702394

Abstract

Purified outer membrane proteins (OMP) of Salmonella minnesota, Re-form, were incorporated into liposomes. These induced in macrophages a chemiluminescence signal identical to that of the intact Re-form. This signal was abolished by preincubation of porin-containing liposomes with purified C1q. Incorporation of isolated OMP into black lipid membranes (BLM) resulted in channel-formation which could not be inhibited by isolated C1q. Additionally, incubation of OMP-containing liposomes with BLM resulted in pore-formation within the BLM. This was amplified when lipid A was present within the liposomes. Preincubation of OMP-containing liposomes with purified C1q abolished pore-formation within the BLM.

摘要

将明尼苏达沙门氏菌Re型的纯化外膜蛋白（OMP）整合到脂质体中。这些蛋白在巨噬细胞中诱导产生的化学发光信号与完整的Re型相同。用纯化的C1q预孵育含孔蛋白的脂质体可消除该信号。将分离的OMP整合到黑色脂质膜（BLM）中会导致通道形成，而分离的C1q无法抑制这种通道形成。此外，将含OMP的脂质体与BLM一起孵育会导致BLM内形成孔。当脂质体中存在脂质A时，这种情况会增强。用纯化的C1q预孵育含OMP的脂质体可消除BLM内的孔形成。