Cloning of a promoter used by sigma H RNA polymerase in Bacillus subtilis.
作者信息
Carter H L, Tatti K M, Moran C P
机构信息
Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, GA 30322.
出版信息
Gene. 1990 Nov 30;96(1):101-5. doi: 10.1016/0378-1119(90)90347-t.
PMID:1702397
Abstract
The secondary RNA polymerase sigma factor sigma H is essential for endospore development in Bacillus subtilis. However, only a few promoters that are used by RNA polymerase containing sigma H (E sigma H) have been identified. We used in vitro transcription of random cloned fragments of B. subtilis chromosomal DNA to identify a promoter that is used by E sigma H. This promoter is active before the onset of sporulation.
摘要
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