Ohmori Sayaka, Arai Tsunenori
School of Fundamental Science and Technology, Graduate School of Science and Technology, Keio University, 3-14-1, Hiyoshi, Kouhoku-ku, Yokohama, 223-8522, Japan.
Lasers Med Sci. 2006 Dec;21(4):213-23. doi: 10.1007/s10103-006-0403-0. Epub 2006 Sep 22.
We studied pulse energy density dependence of two distinctive clinical photosensitizers, Porfimer sodium and Talaporfin sodium, in terms of oxygen consumption, photodegradation in these photosensitizer solutions, and rat prostate cancer cell line photocytotoxicity. The transient transmittances during the pulsed irradiation to these photosensitizer solutions were measured with the pulse energy densities ranging from 0.31 to 31 mJ/cm2. We revealed that Talaporfin sodium was easier to produce absorption saturation than Porfimer sodium. The significant suppression of Talaporfin sodium mediated oxygen consumption, photodegradation, and photocytotoxicity which were observed with pulse energy densities increasing from 0.5 to 10 mJ/cm2. This result could be mainly attributed to absorption saturation. On the other hand, Porfimer sodium did not display significant absorption saturation with the pulse energy densities ranging from 0.31 to 31 mJ/cm2. The photodegradation mechanism for Porfimer sodium changed at high pulse energy density. This phenomenon might accelerate the photodegradation and cause the photocytotoxicity suppression.
我们从氧气消耗、这些光敏剂溶液中的光降解以及大鼠前列腺癌细胞系的光细胞毒性方面，研究了两种不同的临床光敏剂——卟吩姆钠和他拉泊芬钠的脉冲能量密度依赖性。在脉冲能量密度范围为0.31至31 mJ/cm²的情况下，测量了对这些光敏剂溶液进行脉冲照射期间的瞬态透过率。我们发现，他拉泊芬钠比卟吩姆钠更容易产生吸收饱和。随着脉冲能量密度从0.5增加到10 mJ/cm²，观察到他拉泊芬钠介导的氧气消耗、光降解和光细胞毒性受到显著抑制。这一结果主要归因于吸收饱和。另一方面，在脉冲能量密度范围为0.31至31 mJ/cm²时，卟吩姆钠未表现出明显的吸收饱和。在高脉冲能量密度下，卟吩姆钠的光降解机制发生了变化。这种现象可能会加速光降解并导致光细胞毒性抑制。
