关于胆红素在自组装磷脂双分子层上吸附的石英晶体微天平研究。

Quartz crystal microbalance studies on bilirubin adsorption on self-assembled phospholipid bilayers.

作者信息

Yang Zhengpeng, Si Shihui, Zhang Chunjing, Song Ge

机构信息

College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China.

出版信息

J Colloid Interface Sci. 2007 Jan 1;305(1):1-6. doi: 10.1016/j.jcis.2006.09.041. Epub 2006 Sep 22.

DOI:10.1016/j.jcis.2006.09.041

PMID:17028005

Abstract

Bilirubin adsorption on self-assembled phospholipid bilayers was studied using quartz crystal microbalance, and factors influencing its adsorption such as pH, temperature, and solution ionic strength were discussed in detail. The results show the amount of adsorbed bilirubin on self-assembled phospholipid bilayers is small at higher temperature and large at higher pH and solution ionic strength, and the adsorption kinetic parameter estimated from the in situ frequency measurement is (1.8+/-0.27)x10(6) M(-1) (mean +/- S.D.). With the present method, the desorption of adsorbed bilirubin caused by human serum albumin and the photoinduced decomposition of adsorbed bilirubin under light illumination were also examined. QCM measurement provides a useful method for monitoring the adsorption/desorption process of bilirubin on self-assembled phospholipid bilayers.

摘要

利用石英晶体微天平研究了胆红素在自组装磷脂双分子层上的吸附情况，并详细讨论了影响其吸附的因素，如pH值、温度和溶液离子强度。结果表明，在较高温度下，自组装磷脂双分子层上吸附的胆红素量较少；在较高pH值和溶液离子强度下，吸附量较大。通过原位频率测量估算的吸附动力学参数为(1.8±0.27)×10⁶ M⁻¹（平均值±标准差）。采用本方法，还研究了人血清白蛋白引起的吸附胆红素的解吸以及光照下吸附胆红素的光诱导分解。石英晶体微天平测量为监测胆红素在自组装磷脂双分子层上的吸附/解吸过程提供了一种有用的方法。