海洋大型藻类上附生的蓝绿颤藻属物种的分子检测。
Molecular detection of epiphytic Acaryochloris spp. on marine macroalgae.
作者信息
Ohkubo Satoshi, Miyashita Hideaki, Murakami Akio, Takeyama Haruko, Tsuchiya Tohru, Mimuro Mamoru
机构信息
Graduate School of Human and Environmental Studies, Kyoto University, Kyoto 606-8501, Japan.
出版信息
Appl Environ Microbiol. 2006 Dec;72(12):7912-5. doi: 10.1128/AEM.01148-06. Epub 2006 Oct 6.
Abstract
A molecular method for detecting the epiphyte community on marine macroalgae was developed by using PCR-denaturing gradient gel electrophoresis. Selective amplification of 16S rRNA gene fragments from either cyanobacteria or algal plastids improved the detection of minor epiphytes. Two phylotypes of Acaryochloris, a chlorophyll d-containing cyanobacterium, were found not only on red macroalgae but also on green and brown macroalgae.
摘要
通过使用聚合酶链反应-变性梯度凝胶电泳技术，开发了一种用于检测大型海洋藻类附生植物群落的分子方法。从蓝细菌或藻类质体中选择性扩增16S rRNA基因片段，提高了对小型附生植物的检测能力。不仅在红色大型藻类上，而且在绿色和棕色大型藻类上都发现了含叶绿素d的蓝细菌——原绿球藻的两种系统型。
相似文献
1
Molecular detection of epiphytic Acaryochloris spp. on marine macroalgae.海洋大型藻类上附生的蓝绿颤藻属物种的分子检测。
Appl Environ Microbiol. 2006 Dec;72(12):7912-5. doi: 10.1128/AEM.01148-06. Epub 2006 Oct 6.
2
Selective detection and phylogenetic diversity of Acaryochloris spp. that exist in association with didemnid ascidians and sponge.选择性检测与共生的齿裂藻属和海绵相关的 Acaryochloris spp. 的存在，并对其进行系统发育多样性分析。
Microbes Environ. 2012;27(3):217-25. doi: 10.1264/jsme2.me11295. Epub 2012 Feb 22.
3
A new chlorophyll d-containing cyanobacterium: evidence for niche adaptation in the genus Acaryochloris.一种新型含叶绿素 d 的蓝细菌：在集胞藻属中适应小生境的证据。
ISME J. 2010 Nov;4(11):1456-69. doi: 10.1038/ismej.2010.67. Epub 2010 May 27.
4
Acinetobacter diversity in environmental samples assessed by 16S rRNA gene PCR-DGGE fingerprinting.通过16S rRNA基因PCR-DGGE指纹图谱评估环境样品中的不动杆菌多样性。
FEMS Microbiol Ecol. 2004 Oct 1;50(1):37-50. doi: 10.1016/j.femsec.2004.05.007.
5
Rapid TaqMan-based quantification of chlorophyll d-containing cyanobacteria in the genus Acaryochloris.基于TaqMan技术对蓝藻科中含叶绿素d的蓝细菌进行快速定量分析。
Appl Environ Microbiol. 2014 May;80(10):3244-9. doi: 10.1128/AEM.00334-14. Epub 2014 Mar 14.
6
Molecular characterization of cyanobacterial diversity in a shallow eutrophic lake.一个浅水富营养化湖泊中蓝藻多样性的分子特征分析
Environ Microbiol. 2005 Mar;7(3):365-77. doi: 10.1111/j.1462-2920.2005.00715.x.
7
Bifidobacterial diversity in human feces detected by genus-specific PCR and denaturing gradient gel electrophoresis.通过属特异性聚合酶链反应和变性梯度凝胶电泳检测人类粪便中的双歧杆菌多样性。
Appl Environ Microbiol. 2001 Feb;67(2):504-13. doi: 10.1128/AEM.67.2.504-513.2001.
8
Detection of the Na(+)-translocating NADH-quinone reductase in marine bacteria using a PCR technique.利用聚合酶链式反应技术检测海洋细菌中的钠转运型NADH-醌还原酶。
Can J Microbiol. 2000 Apr;46(4):325-32.
9
A comparison of DNA- and RNA-based clone libraries from the same marine bacterioplankton community.来自同一海洋浮游细菌群落的基于DNA和基于RNA的克隆文库的比较。
FEMS Microbiol Ecol. 2005 Feb 1;51(3):341-52. doi: 10.1016/j.femsec.2004.09.012. Epub 2004 Nov 11.
10
16S ribosomal RNA-based methods to monitor changes in the hindgut bacterial community of piglets after oral administration of Lactobacillus sobrius S1.基于16S核糖体RNA的方法监测口服嗜 sobrius 乳杆菌S1后仔猪后肠细菌群落的变化。
Anaerobe. 2008 Apr;14(2):78-86. doi: 10.1016/j.anaerobe.2007.12.004. Epub 2008 Jan 5.
引用本文的文献
1
An indigenous microalgal pool containing Klebsormidium sp. K39 as a stable and efficacious biotechnological strategy for Escherichia coli removal in urban wastewater treatment.一个含有鞘毛藻属K39的本地微藻库，作为城市污水处理中去除大肠杆菌的一种稳定且有效的生物技术策略。
J Sci Food Agric. 2025 Jan 30;105(2):1288-1297. doi: 10.1002/jsfa.13918. Epub 2024 Sep 23.
2
Symbioses of Cyanobacteria in Marine Environments: Ecological Insights and Biotechnological Perspectives.海洋环境中的蓝藻共生体：生态见解和生物技术视角。
Mar Drugs. 2021 Apr 16;19(4):227. doi: 10.3390/md19040227.
3
A niche for cyanobacteria producing chlorophyll f within a microbial mat.微生物垫中产生叶绿素f的蓝细菌的生态位。
ISME J. 2017 Oct;11(10):2368-2378. doi: 10.1038/ismej.2017.98. Epub 2017 Jun 16.
4
Rapid TaqMan-based quantification of chlorophyll d-containing cyanobacteria in the genus Acaryochloris.基于TaqMan技术对蓝藻科中含叶绿素d的蓝细菌进行快速定量分析。
Appl Environ Microbiol. 2014 May;80(10):3244-9. doi: 10.1128/AEM.00334-14. Epub 2014 Mar 14.
5
Unusual symbiotic cyanobacteria association in the genetically diverse intertidal marine sponge Hymeniacidon perlevis (Demospongiae, Halichondrida).在遗传多样性的潮间带海洋海绵 Hymeniacidon perlevis（寻常海绵纲，矾海绵目）中存在不寻常的共生蓝细菌共生关系。
PLoS One. 2012;7(12):e51834. doi: 10.1371/journal.pone.0051834. Epub 2012 Dec 14.
6
Mining genomes of marine cyanobacteria for elements of zinc homeostasis.挖掘海洋蓝细菌基因组中的锌稳态元素。
Front Microbiol. 2012 Apr 11;3:142. doi: 10.3389/fmicb.2012.00142. eCollection 2012.
7
Selective detection and phylogenetic diversity of Acaryochloris spp. that exist in association with didemnid ascidians and sponge.选择性检测与共生的齿裂藻属和海绵相关的 Acaryochloris spp. 的存在，并对其进行系统发育多样性分析。
Microbes Environ. 2012;27(3):217-25. doi: 10.1264/jsme2.me11295. Epub 2012 Feb 22.
8
Genome fluctuations in cyanobacteria reflect evolutionary, developmental and adaptive traits.蓝藻基因组的波动反映了其进化、发育和适应特征。
BMC Evol Biol. 2011 Jun 30;11:187. doi: 10.1186/1471-2148-11-187.
9
Endolithic chlorophyll d-containing phototrophs.内共生叶绿素 d 含量光合生物。
ISME J. 2011 Jun;5(6):1072-6. doi: 10.1038/ismej.2010.195. Epub 2010 Dec 16.
10
Epibiosis of oxygenic phototrophs containing chlorophylls a, b, c, and d on the colonial ascidian Cystodytes dellechiajei.叶绿素 a、b、c 和 d 含氧光合生物在群体海鞘 Cystodytes dellechiajei 上的附生现象。
Microb Ecol. 2011 Jan;61(1):13-9. doi: 10.1007/s00248-010-9694-6. Epub 2010 Jun 8.
本文引用的文献
1
Testing of primers for the study of cyanobacterial molecular diversity by DGGE.用于通过变性梯度凝胶电泳（DGGE）研究蓝藻分子多样性的引物测试。
J Microbiol Methods. 2006 Jun;65(3):542-50. doi: 10.1016/j.mimet.2005.09.017. Epub 2005 Nov 14.
2
Chlorophyll d in an epiphytic cyanobacterium of red algae.红藻附生蓝细菌中的叶绿素d。
Science. 2004 Mar 12;303(5664):1633. doi: 10.1126/science.1095459.
3
Spatial analysis of archaeal community structure in grassland soil.草原土壤中古菌群落结构的空间分析
Appl Environ Microbiol. 2003 Dec;69(12):7420-9. doi: 10.1128/AEM.69.12.7420-7429.2003.
4
Cyanobacterial diversity in natural and artificial microbial mats of Lake Fryxell (McMurdo Dry Valleys, Antarctica): a morphological and molecular approach.弗里克塞尔湖（南极洲麦克默多干谷）天然和人工微生物席中的蓝藻多样性：形态学和分子学方法
Appl Environ Microbiol. 2003 Sep;69(9):5157-69. doi: 10.1128/AEM.69.9.5157-5169.2003.
5
Microbial characterization of biofilms in domestic drains and the establishment of stable biofilm microcosms.家庭排水管道生物膜的微生物特性及稳定生物膜微观世界的建立。
Appl Environ Microbiol. 2003 Jan;69(1):177-85. doi: 10.1128/AEM.69.1.177-185.2003.
6
Long-term compositional changes after transplant in a microbial mat cyanobacterial community revealed using a polyphasic approach.采用多相方法揭示移植后微生物席蓝藻群落的长期组成变化。
Environ Microbiol. 2001 Jan;3(1):53-62. doi: 10.1046/j.1462-2920.2001.00159.x.
7
PCR primers to amplify 16S rRNA genes from cyanobacteria.用于从蓝细菌中扩增16S rRNA基因的PCR引物。
Appl Environ Microbiol. 1997 Aug;63(8):3327-32. doi: 10.1128/aem.63.8.3327-3332.1997.
8
Denaturing gradient gel electrophoresis profiles of 16S rRNA-defined populations inhabiting a hot spring microbial mat community.栖息于温泉微生物席群落的16S rRNA界定菌群的变性梯度凝胶电泳图谱。
Appl Environ Microbiol. 1996 Feb;62(2):340-6. doi: 10.1128/aem.62.2.340-346.1996.
9
Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA.通过对聚合酶链反应扩增的编码16S rRNA的基因进行变性梯度凝胶电泳分析来剖析复杂微生物群落。
Appl Environ Microbiol. 1993 Mar;59(3):695-700. doi: 10.1128/aem.59.3.695-700.1993.
10
Phylogenetic relationships of Thiomicrospira species and their identification in deep-sea hydrothermal vent samples by denaturing gradient gel electrophoresis of 16S rDNA fragments.硫微螺菌属物种的系统发育关系及其在深海热液喷口样品中通过16S rDNA片段变性梯度凝胶电泳进行的鉴定。
Arch Microbiol. 1995 Sep;164(3):165-72. doi: 10.1007/BF02529967.
Zotero 插件