Marriott D R, Wilkin G P, Wood J N
Department of Biochemistry, Imperial College of Science, Technology and Medicine, London, England.
J Neurochem. 1991 Jan;56(1):259-65. doi: 10.1111/j.1471-4159.1991.tb02590.x.
Addition of substance P (SP) to astrocytes cultured from rat neonatal spinal cord evoked a time- and concentration-dependent increase in the accumulation of phosphoinositol and the release of prostaglandin (PG) D2 and PGE2. Both basal and stimulated releases were reduced to similar levels by indomethacin. In contrast, astrocytes cultured from cerebral cortex and cerebellum showed no SP-stimulated increase in phosphoinositol accumulation or release of PGs. Release of PGD2 and PGE2 was, however, stimulated by the calcium ionophore A23187, and both phosphoinositol accumulation and PG release were stimulated from cortical astrocytes incubated in the presence of serum. The results from this study suggest that SP-stimulated phosphoinositol accumulation and release of PGs from cultured rat neonatal astrocytes are regionally specialised in favour of cells derived from spinal cord.
将P物质（SP）添加到从新生大鼠脊髓培养的星形胶质细胞中，可引起磷酸肌醇积累以及前列腺素（PG）D2和PGE2释放呈时间和浓度依赖性增加。吲哚美辛可将基础释放和刺激释放均降低至相似水平。相比之下，从大脑皮层和小脑培养的星形胶质细胞未显示出SP刺激的磷酸肌醇积累增加或PG释放。然而，钙离子载体A23187可刺激PGD2和PGE2的释放，并且在血清存在下培养的皮层星形胶质细胞的磷酸肌醇积累和PG释放均受到刺激。这项研究的结果表明，SP刺激的培养新生大鼠星形胶质细胞中磷酸肌醇积累和PG释放具有区域特异性，有利于源自脊髓的细胞。
