Unger Volker, Grosse-Siestrup Christian, Groneberg David A
Department of Experimental Animal Sciences, Charité - Universitaetsmedizin Berlin, Free University and Humboldt University, D-13353 Berlin, Germany.
Physiol Meas. 2006 Nov;27(11):1167-75. doi: 10.1088/0967-3334/27/11/009. Epub 2006 Sep 20.
Isolated porcine kidneys are commonly used to study physiological and pathophysiological aspects of renal homeostasis but standardized evaluation procedures of renal function in this model do not exist so far. A double-logarithmical nomogram is established for filtration and reabsorption functions in isolated and hemoperfused porcine kidneys using different perfusion settings. Model validity was demonstrated by the levels of urine flow and sodium excretion showing expected alteration levels of lowering in the ADH-group and increasing in the furosemide-group of isolated kidneys. Creatinine-clearance values were in constant ranges within each specific perfusion group as indicated by the nomogram procedure. The present studies used a nomogram method to analyze the effects of different renal perfusion settings in a porcine model of kidney perfusion. The method may be of use to differentiate various kidney perfusion parameters both at the experimental and clinical levels.
孤立猪肾常用于研究肾脏内环境稳定的生理和病理生理方面，但目前该模型中尚无标准化的肾功能评估程序。利用不同的灌注设置，为孤立和血液灌注的猪肾的滤过和重吸收功能建立了双对数列线图。通过尿量和钠排泄水平证明了模型的有效性，显示在抗利尿激素组中孤立肾的预期降低水平和在呋塞米组中增加的水平。根据列线图程序，肌酐清除值在每个特定灌注组内处于恒定范围内。本研究使用列线图方法分析不同肾脏灌注设置对猪肾脏灌注模型的影响。该方法可能有助于在实验和临床水平上区分各种肾脏灌注参数。
