Lee Dong Hwan, Kim Jung Mo, Kang Seong Woo, Lee Ji Won, Kim Seung Wook
Department of Chemical and Biological Engineering, Korea University, Anam-dong, Sungbuk-ku, Seoul, Korea.
Biotechnol Lett. 2006 Dec;28(23):1965-9. doi: 10.1007/s10529-006-9181-9. Epub 2006 Sep 22.
Lipase was pretreated with soybean oil in order to allow fatty acids to bond to the active site before immobilization. This pretreated lipase exhibited steric hindrance around the active site such that during immobilization, covalent bonds were formed between the carrier and the lipase region far from the active site. The activity of the pretreated lipase immobilized covalently on a silica gel was 530 U/g-matrix, which is 16 times higher than that of the immobilized non-pretreated lipase. In addition, the immobilized lipase activity was maintained at levels exceeding 90% of its original activity after 10 reuses.
脂肪酶用大豆油进行预处理，以便在固定化之前使脂肪酸与活性位点结合。这种预处理后的脂肪酶在活性位点周围表现出空间位阻，使得在固定化过程中，载体与远离活性位点的脂肪酶区域之间形成共价键。共价固定在硅胶上的预处理脂肪酶的活性为530 U/g载体，这比未预处理的固定化脂肪酶的活性高16倍。此外，固定化脂肪酶在重复使用10次后，其活性保持在超过原始活性90%的水平。
