Remigereau Marie-Stanislas, Robin Odile, Siljak-Yakovlev Sonia, Sarr Aboubakry, Robert Thierry, Langin Thierry
Institut de Biotechnologies des Plantes (UMR 8618), Université Paris-Sud, Bat.630, 91405, Orsay Cedex, France.
Genetica. 2006 Sep-Nov;128(1-3):205-16. doi: 10.1007/s10709-005-5703-y.
Miniature-inverted repeat transposable elements (MITEs) are abundantly repeated in plant genomes and are especially found in genic regions where they could contribute regulatory elements for gene expression. We describe with molecular and cytological tools the first MITE family reported in pearl millet: Tuareg. It was initially detected in the pearl millet ortholog of Teosinte-branched1, an important developmental gene involved in the domestication of maize. The Tuareg family was amplified recently in the pearl millet genome and elements were found more abundant in wild than in domesticated plants. We found that they shared similarity in their terminal repeats with the previously described mPIF MITEs and that they are also present in other Pennisetum species, in maize and more distantly related grasses. The Tuareg family may be part of MITEs activated by PIF-like transposases and it could have been mobile since pearl millet domestication.
微型反向重复转座元件（MITEs）在植物基因组中大量重复出现，尤其在基因区域中发现，它们可能为基因表达贡献调控元件。我们使用分子和细胞学工具描述了珍珠粟中报道的首个MITE家族：图阿雷格（Tuareg）。它最初在玉米驯化过程中涉及的重要发育基因——玉米分枝1（Teosinte-branched1）的珍珠粟直系同源基因中被检测到。图阿雷格家族最近在珍珠粟基因组中扩增，并且在野生植物中比在驯化植物中发现的元件更为丰富。我们发现它们的末端重复序列与先前描述的mPIF MITEs具有相似性，并且它们也存在于其他狼尾草属物种、玉米以及亲缘关系更远的禾本科植物中。图阿雷格家族可能是由类PIF转座酶激活的MITEs的一部分，并且自珍珠粟驯化以来它可能一直具有移动性。
