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一种含五肽重复序列（PPR）蛋白基因的新型拟南芥半致死突变体的分离与鉴定。

Isolation and characterization of a novel semi-lethal Arabidopsis thaliana mutant of gene for pentatricopeptide (PPR) repeat-containing protein.

作者信息

Kocábek Tomás, Repková Jana, Dudová Markéta, Hoyerová Klára, Vrba Lukás

机构信息

Institute of Plant Molecular Biology, Biological Centre of the Academy of Sciences of the Czech Republic, Branisovská 31, CZ-370 05, Ceské Budejovice, Czech Republic.

出版信息

Genetica. 2006 Sep-Nov;128(1-3):395-407. doi: 10.1007/s10709-006-7518-x.

DOI:10.1007/s10709-006-7518-x
PMID:17028967
Abstract

A novel Arabidopsis thaliana mutant of one member of the pentatricopeptide repeat (PPR) gene family has been identified among T-DNA insertion lines. Tagging of the At1g53330 gene caused the appearance of a semi-lethal mutation with a complex phenotypic expression from embryo lethality associated with the abnormal pattern of cell division during globular to heart transition to fertile plants with just subtle phenotypic changes. The PPR protein At1g53330.1 was predicted to be targeted to mitochondria by TargetP and MitoProt programs. Complementation analysis confirmed that the phenotype is a result of a single T-DNA integration. A thorough functional analysis of this mutant aimed at finding a particular organelle target of At1g53330.1 protein will follow.

摘要

在T-DNA插入系中鉴定出了拟南芥五肽重复序列（PPR）基因家族一个成员的新型突变体。At1g53330基因的标签导致了一个半致死突变的出现，其具有复杂的表型表达，从与球状胚向心形胚转变过程中细胞分裂异常模式相关的胚胎致死到仅有细微表型变化的可育植株。通过TargetP和MitoProt程序预测PPR蛋白At1g53330.1定位于线粒体。互补分析证实该表型是单个T-DNA整合的结果。接下来将对该突变体进行全面的功能分析，旨在找到At1g53330.1蛋白的特定细胞器靶点。

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