Goel H C, Prakash H, Ali A, Bala M
Department of Microbiology, C. C. S. University, Meerut, India.
Mol Cell Biochem. 2007 Jan;295(1-2):93-103. doi: 10.1007/s11010-006-9277-5. Epub 2006 Oct 7.
Aqueous extract of Podophyllum hexandrum (RP-1), which has been reported to render more than 82% survival against whole body lethal (10 Gy) gamma-irradiation in mice, was further investigated for its immunomodulatory potential. In this study, no significant change could be scored in peritoneal macrophages survival up to 8th day after whole body irradiation. RP-1 treatment (200 mg/kg body weight, i.p.) alone or 2 h before whole body irradiation enhanced macrophage survival significantly (p<0.05) as compared to irradiated control mice. In irradiated animals, there was significant (p<0.01) reduction in splenocyte survival and proliferation as revealed by 3H-TdR method. RP-1 treatment (200 mg/kg) alone or 2 h before irradiation countered the decrease in survival of splenocytes and proliferation significantly (p<0.05) as compared to irradiated control group. Whole body irradiation also significantly (p<0.05) reduced the population of CD4+ and CD8+ T cells and bone marrow GM-CFU at 24 h and 72 h post-irradiation intervals, respectively, as compared to unirradiated control. RP-1 treatment 2 h before whole body irradiation countered the decrease in CD4+ and CD8+ T cells populations and CGM-CFU. Nitric oxide free radicals generation was enhanced significantly (p<0.05) in the supernatant of peritoneal macrophage cultures exposed to 2 Gy gamma radiation ex vivo in comparison to unirradiated control, which was reduced by pre-irradiation (-2 h) administration of RP-1. Whole body irradiation (10 Gy) also reduced the serum titres of IL-3, IL-1 and various IgG isotypes observed at different post-irradiation time interval. RP-1 treatment alone or before whole body irradiation countered radiation induced decrease in the titre of IL-1, IL-3 and IgG's in the serum of mice. These findings indicate immunostimulatory potential of RP-1.
鬼臼（RP - 1）水提取物据报道可使小鼠在全身致死剂量（10 Gy）γ射线照射下的存活率超过82%，本研究进一步探讨了其免疫调节潜力。在本研究中，全身照射后第8天内，腹膜巨噬细胞存活率无显著变化。与照射对照组小鼠相比，单独给予RP - 1处理（200 mg/kg体重，腹腔注射）或在全身照射前2小时给予该处理，可显著提高巨噬细胞存活率（p<0.05）。通过3H - TdR方法发现，照射动物的脾细胞存活率和增殖率显著降低（p<0.01）。与照射对照组相比，单独给予RP - 1处理（200 mg/kg）或在照射前2小时给予该处理可显著对抗脾细胞存活率和增殖率的降低（p<0.05）。与未照射对照组相比，全身照射在照射后24小时和72小时时还分别显著降低了CD4 +和CD8 + T细胞以及骨髓GM - CFU的数量。全身照射前2小时给予RP - 1处理可对抗CD4 +和CD8 + T细胞数量以及CGM - CFU的减少。与未照射对照组相比，体外暴露于2 Gy γ射线的腹膜巨噬细胞培养上清液中一氧化氮自由基的生成显著增强（p<0.05），而照射前（-2小时）给予RP - 1可使其减少。全身照射（10 Gy）还降低了在不同照射后时间间隔观察到的IL - 3、IL - 1和各种IgG同种型的血清滴度。单独给予RP - 1处理或在全身照射前给予该处理可对抗辐射诱导的小鼠血清中IL - 1、IL - 3和IgG滴度的降低。这些发现表明RP - 1具有免疫刺激潜力。
