Prakash Hridayesh, Ali Arif, Bala Madhu, Goel Harish Chandra
Department of Radiation Biology, Institute of Nuclear Medicine and Allied Sciences, Brig. S. K. Mazumdar Marg, Delhi, India.
J Pharm Pharm Sci. 2005 Apr 30;8(1):107-14.
Down-regulation of lipopolysaccharide (LPS) induced hyper-inflammatory response by non-toxic pharmacological agents acquires paramount importance for countering bacterial sepsis. Anti-inflammatory potential of aqueous extract of Podophyllum hexandrum, a plant well documented in Ayurvedic literature for various therapeutic purposes, was investigated.
In vivo studies were performed on Balb/c mice pre-treated with supra-lethal dose of LPS endotoxin (E.coli 055:B5) with or without treatment with P. hexandrum extract (RP-1). Mouse peritoneal macrophage cultures were used to understand ex vivo effects of RP-1 on LPS generated nitric oxide (NO), secretion of IFN-gamma, IL-6 and TNF-alpha. Griess assay and sandwich ELISA method were used to quantify inducible NO and cytokines respectively.
Minimal dose of LPS that rendered 100% mortality to mice was found to be 450 microg/kg b.w. Administration of RP-1 (200 mg/kg b.w., i.p.) one hour before lethal LPS treatment (0.5 mg/kg b.w.) rendered maximum (78%) survival. Ex vivo study revealed that RP-1 (50 microg/ml) treatment to peritoneal macrophages inhibited LPS (5 microg/ml) induced nitrite generation to 37%, IFN-gamma secretion to 5%, IL-6 secretion to 50% and TNF-alpha secretion to 50 % of LPS treated control values.
This study has demonstrated anti-inflammatory potential of aqueous extract of P. hexandrum.
利用无毒药理剂下调脂多糖（LPS）诱导的过度炎症反应对于对抗细菌性败血症至关重要。研究了鬼臼（Podophyllum hexandrum）水提取物的抗炎潜力，该植物在阿育吠陀文献中有多种治疗用途的详细记载。
对预先用超致死剂量的LPS内毒素（大肠杆菌055:B5）处理的Balb/c小鼠进行体内研究，部分小鼠同时或不同时接受鬼臼提取物（RP-1）治疗。使用小鼠腹腔巨噬细胞培养物来了解RP-1对LPS产生的一氧化氮（NO）、IFN-γ、IL-6和TNF-α分泌的体外作用。分别采用Griess法和夹心ELISA法对诱导型NO和细胞因子进行定量。
发现使小鼠100%死亡的最小LPS剂量为450微克/千克体重。在致死性LPS处理（0.5毫克/千克体重）前1小时腹腔注射RP-1（200毫克/千克体重）可使存活率最高达到78%。体外研究表明，用RP-1（50微克/毫升）处理腹腔巨噬细胞可使LPS（5微克/毫升）诱导的亚硝酸盐生成抑制至LPS处理对照组值的37%，IFN-γ分泌抑制至5%，IL-6分泌抑制至50%，TNF-α分泌抑制至50%。
本研究证明了鬼臼水提取物的抗炎潜力。
