Yamanashi Y, Kakiuchi T, Mizuguchi J, Yamamoto T, Toyoshima K
Department of Oncology, University of Tokyo, Japan.
Science. 1991 Jan 11;251(4990):192-4. doi: 10.1126/science.1702903.
Antigen is thought to cross-link membrane-bound immunoglobulins (Igs) of B cells, causing proliferation and differentiation or the inhibition of growth. Protein tyrosine kinases are probably involved in signal transduction for cell proliferation and differentiation. The Src-like protein tyrosine kinase Lyn is expressed preferentially in B cells. The Lyn protein and its kinase activity could be coimmunoprecipitated with IgM from detergent lysates. Cross-linking of membrane-bound IgM induced a rapid increase in tyrosine phosphorylation of at least ten distinct proteins of B cells. Thus, Lyn is physically associated with membrane-bound IgM, and is suggested to participate in antigen-mediated signal transduction.
抗原被认为可交联B细胞膜结合免疫球蛋白（Ig），导致增殖和分化或生长抑制。蛋白酪氨酸激酶可能参与细胞增殖和分化的信号转导。Src样蛋白酪氨酸激酶Lyn优先在B细胞中表达。Lyn蛋白及其激酶活性可从去污剂裂解物中与IgM共免疫沉淀。膜结合IgM的交联诱导B细胞至少十种不同蛋白的酪氨酸磷酸化迅速增加。因此，Lyn与膜结合IgM在物理上相关联，并被认为参与抗原介导的信号转导。
