Ren Chen-chun, Miao Xu-hong, Yang Bin, Zhao Lei, Sun Rui, Song Wen-qin
Department of Genetics, the Central Hospital of Gynecology and Obstetric, Tianjin, 300052 PR China.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2006 Oct;23(5):565-7.
To explore the methylation status of 5' CpG island of fragile histidine triad (FHIT) gene in plasma and the expression of FHIT protein in cancer tissue of cervical cancer patients.
Methylation-specific PCR (MS-PCR) was employed to examine methylation of FHIT gene in 151 plasma samples before treatment. The immunohistochemistry was used to the expression of FHIT protein in cancer tissues.
CpG island methylation of FHIT was detected in 31.13% of plasma samples. The expression of FHIT protein was decreased or discarded in 59.60% of cervical cancer tissues. Among them 47.78% was included in methylation positive samples.
CpG island methylation of FHIT gene in plasma plays an important role on cervical cancer, which results in decreased expression of FHIT protein. It can be used to diagnose and evaluate the effect of treatment to cervical cancers.
探讨宫颈癌患者血浆中脆性组氨酸三联体（FHIT）基因5'CpG岛甲基化状态及癌组织中FHIT蛋白表达情况。
采用甲基化特异性PCR（MS-PCR）检测151例治疗前血浆样本中FHIT基因甲基化情况，采用免疫组织化学法检测癌组织中FHIT蛋白表达情况。
31.13%的血浆样本检测到FHIT基因CpG岛甲基化。59.60%的宫颈癌组织中FHIT蛋白表达降低或缺失，其中47.78%包含在甲基化阳性样本中。
血浆中FHIT基因CpG岛甲基化在宫颈癌发生中起重要作用，导致FHIT蛋白表达降低，可用于宫颈癌的诊断及疗效评估。
