Lymangrover J, Pearlmutter A F, Franco-Saenz R, Saffran M
J Clin Endocrinol Metab. 1975 Oct;41(4):697-706. doi: 10.1210/jcem-41-4-697.
Trans-membrane potentials and steroidogenesis were measured in superfused slices of non-tumor and neoplastic human adrenocortical tissue. Non-tumor tissue was obtained at the time for renal transplant or from tissue removed along with tumors. Non-tumor human adrenocortical tissue had electrophysiological and steroidogenic properties similar to those of the rat and rabbit. In normal medium ACTH stimulated steroidogenesis but had no effect on the membrane potential. In K+-free medium, the cells hyperpolarized, and subsequent addition of ACTH caused depolarization. Trans-membrane potentials of adrenocortical tumors were lower than those of non-tumor cells. Ommission of K+ from the medium caused hyperpolairzation of the tumor cells, but the trans-membrane potentials did not reach the values of hyperpolarized non-tumor cells. ACTH, added to the K+-free medium, caused little or no change in membrane potential of tumor cells except in one case of a virilizing adenoma, which responded very much like non-tumor tissue. Except for the virilizing adenoma, tumor tissue slices produced little or no detectable fluorogenic steroid, even in the presence of large amounts of ACTH or cyclic AMP. The virilizing adenoma responded with increased steroidogensis to ACTH and cyclic AMP.
在非肿瘤性和肿瘤性人类肾上腺皮质组织的灌流切片中测量跨膜电位和类固醇生成。非肿瘤组织取自肾移植时或与肿瘤一并切除的组织。非肿瘤性人类肾上腺皮质组织具有与大鼠和兔子相似的电生理和类固醇生成特性。在正常培养基中，促肾上腺皮质激素（ACTH）刺激类固醇生成，但对膜电位没有影响。在无钾培养基中，细胞超极化，随后添加ACTH导致去极化。肾上腺皮质肿瘤的跨膜电位低于非肿瘤细胞。培养基中去除钾导致肿瘤细胞超极化，但跨膜电位未达到超极化非肿瘤细胞的值。添加到无钾培养基中的ACTH除了在一例男性化腺瘤中导致肿瘤细胞膜电位几乎没有变化或没有变化外，该男性化腺瘤的反应与非肿瘤组织非常相似。除男性化腺瘤外，肿瘤组织切片即使在存在大量ACTH或环磷酸腺苷（cAMP）的情况下也几乎不产生或不产生可检测到的荧光类固醇。男性化腺瘤对ACTH和cAMP的反应是类固醇生成增加。
