LeBeau Marc A, Montgomery Madeline A, Morris-Kukoski Cynthia, Schaff Jason E, Deakin Anna
Chemistry Unit, FBI Laboratory, Federal Bureau of Investigation, 2501 Investigation Parkway, Quantico, VA 22135, United States.
Forensic Sci Int. 2007 Jul 4;169(2-3):152-6. doi: 10.1016/j.forsciint.2006.08.007. Epub 2006 Oct 9.
This study was designed to supplement previous studies that documented in vitro production of gamma-hydroxybutyrate (GHB) in urine samples. Urine samples were provided by subjects who reported that they had never used GHB (n=31). The specimens were stored under standard conditions of refrigeration (5 degrees C) without any preservatives added. All specimens were repeatedly analyzed for the presence of endogenous GHB over a 6-month period using a previously reported headspace GC-MS method. Significant elevations in GHB were observed in many of the urine samples as storage time increased. As a result, the in vitro production of GHB may increase the apparent GHB concentrations in urine during storage. This potential for an artificial increase in GHB concentration must be appreciated when establishing the threshold between endogenous and exogenous concentrations of GHB.
本研究旨在补充先前的研究，这些研究记录了尿液样本中γ-羟基丁酸（GHB）的体外生成情况。尿液样本由报告称从未使用过GHB的受试者提供（n = 31）。样本在标准冷藏条件（5摄氏度）下储存，未添加任何防腐剂。使用先前报道的顶空气相色谱-质谱法，在6个月的时间内对所有样本反复分析内源性GHB的存在情况。随着储存时间的增加，许多尿液样本中观察到GHB显著升高。因此，GHB的体外生成可能会在储存期间增加尿液中GHB的表观浓度。在确定GHB内源性浓度和外源性浓度之间的阈值时，必须认识到这种人为增加GHB浓度的可能性。
