Rostami A, Gregorian S K
Department of Neurology, University of Pennsylvania, School of Medicine, Philadelphia 19104.
Cell Immunol. 1991 Feb;132(2):433-41. doi: 10.1016/0008-8749(91)90040-i.
We have recently described the clinical and pathological features of experimental autoimmune neuritis (EAN) in Lewis rats inoculated with varying doses of a synthetic peptide corresponding to the amino acid residues 53-78 of bovine P2 protein (SP-26). Immunization with this synthetic peptide was able to induce severe clinical and pathological characteristics of EAN. We are now reporting that, SP-26 T cell lines derived from spleen and lymph node cell populations of such immunized rats, upon being triggered by SP-26, can adoptively transfer severe clinical and histological signs of EAN to naive syngeneic recipients. The disease appears 7-8 days postinoculation of the cells and persist 5-10 days. The pathological features were indistinguishable from SP-26-induced active EAN which appears 12-15 days after sensitization. Examination of the surface phenotype of the cells that were used for the passive transfer of EAN by FACS analysis, showed majority of the cells to be CD4+, Ia+ cells.
我们最近描述了用不同剂量的对应于牛P2蛋白氨基酸残基53 - 78的合成肽（SP - 26）接种的Lewis大鼠实验性自身免疫性神经炎（EAN）的临床和病理特征。用这种合成肽免疫能够诱导EAN的严重临床和病理特征。我们现在报告，从这种免疫大鼠的脾脏和淋巴结细胞群体中获得的SP - 26 T细胞系，在受到SP - 26触发后，能够将EAN的严重临床和组织学体征过继转移给同基因的未致敏受体。在接种细胞后7 - 8天出现疾病，并持续5 - 10天。其病理特征与致敏后12 - 15天出现的SP - 26诱导的活动性EAN无法区分。通过流式细胞术分析用于EAN被动转移的细胞的表面表型，结果显示大多数细胞为CD4 +、Ia +细胞。
