Asaka Mitsuru, Ohta Kazumasa, Muramatsu Takashi, Kurokawa Mai, Kizaki Harutoshi, Hashimoto Sadamitsu, Shimono Masaki
Department of Pathology, Tokyo Dental College, Chiba, Japan.
Arch Histol Cytol. 2006 Sep;69(3):181-8. doi: 10.1679/aohc.69.181.
The present study investigated the expression and distribution of osteopontin in the mouse major salivary glands. The level of osteopontin expression in the mouse submandibular gland was higher (12.7-fold) than that in parotid and sublingual glands at the mRNA level. By Western blot analysis, intense positive bands were seen at the predicted molecular mass (about 55 kDa) in all the major salivary glands, while an approximately 30 kDa band of osteopontin was detected only in the submandibular gland. Indirect immunofluorescent and immuno-electron microscopy analyses demonstrated the localization of osteopontin in the luminal (apical) membranes of acinar cells in all the salivary glands. Osteopontin was also localized at the lumen of acini in the submandibular gland. These results suggest that the expression of osteopontin in the submandibular gland is different from that in the parotid and sublingual glands and that osteopontin may be degraded in the mouse submandibular gland.
本研究调查了骨桥蛋白在小鼠主要唾液腺中的表达和分布。在mRNA水平上，小鼠下颌下腺中骨桥蛋白的表达水平高于腮腺和舌下腺（高12.7倍）。通过蛋白质免疫印迹分析，在所有主要唾液腺中均可见到预测分子量（约55 kDa）处的强阳性条带，而仅在下颌下腺中检测到约30 kDa的骨桥蛋白条带。间接免疫荧光和免疫电子显微镜分析表明，骨桥蛋白定位于所有唾液腺腺泡细胞的管腔（顶端）膜中。骨桥蛋白也定位于下颌下腺腺泡的管腔中。这些结果表明，下颌下腺中骨桥蛋白的表达与腮腺和舌下腺不同，并且骨桥蛋白可能在小鼠下颌下腺中被降解。
