Fischer E, Delibrias C, Kazatchkine M D
INSERM U28, Hôpital Broussais, Paris, France.
J Immunol. 1991 Feb 1;146(3):865-9.
The expression of CR2 (the C3dg/EBV receptor, CD21) on normal human T lymphocytes was investigated using purified peripheral blood T cells and indirect immunofluorescence with biotinylated anti-CR2 mAb and streptavidin-phycoerythrin. Thirty to 40% of normal peripheral blood T lymphocytes expressed CR2 Ag. The cells expressed three nonoverlapping epitopes of CR2. The specificity of the staining for CR2 epitopes was demonstrated by the ability of unlabeled anti-CR2 mAb but not of anti-CR1 mAb of the same isotype to compete for the binding of biotinylated anti-CR2 mAb to T cells. The intensity of staining of T lymphocytes with anti-CR2 mAb was approximately 10-fold lower than that of peripheral blood B cells. CR2 was immunoprecipitated from purified T lymphocytes as a single protein of apparent Mr 145,000. The presence of CR2 on normal human T lymphocytes suggests that the receptor may modulate the function of T cells in the immune response and the susceptibility of the cells to infection by lymphocytotropic viruses.
利用纯化的外周血T细胞以及生物素化抗CR2单克隆抗体和链霉亲和素-藻红蛋白进行间接免疫荧光，研究了正常人T淋巴细胞上CR2（C3dg/EBV受体，CD21）的表达。30%至40%的正常外周血T淋巴细胞表达CR2抗原。这些细胞表达CR2的三个不重叠表位。未标记的抗CR2单克隆抗体（而非相同亚型的抗CR1单克隆抗体）能够竞争生物素化抗CR2单克隆抗体与T细胞的结合，从而证明了CR2表位染色的特异性。抗CR2单克隆抗体对T淋巴细胞的染色强度比外周血B细胞低约10倍。从纯化的T淋巴细胞中免疫沉淀出的CR2是一种表观分子量为145,000的单一蛋白质。正常人T淋巴细胞上存在CR2表明该受体可能在免疫反应中调节T细胞的功能以及细胞对嗜淋巴细胞病毒感染的易感性。
