Miranda S, Litwin S, Barrientos G, Szereday L, Chuluyan E, Bartho J S, Arck P C, Blois S M
Department of Microbiology, Biotechnology and Immunology, IDEHU (CONICET-University of Buenos Aires), Buenos Aires, Argentina.
Scand J Immunol. 2006 Nov;64(5):493-9. doi: 10.1111/j.1365-3083.2006.01841.x.
The fetal-placental unit is a semi-allograft and immunological recognition of pregnancy, together with the subsequent response of the maternal immune system, is necessary for a successful pregnancy. Dendritic cells (DC) show a biological plasticity that confers them special characteristics regulating both immunity and tolerance. Therapy employing DC proved to diminish the abortion in the DBA/2J-mated CBA/J females; however, the underlying mechanisms remain unknown. Here, we evaluated whether DC therapy influences the presence of immunoregulatory populations of cells at the fetal-maternal interface. To address this hypothesis, we analysed the pregnancy-protective CD8, gammadelta cell populations as well as transforming growth factor (TGF)-beta1 and progesterone-induced blocking factor (PIBF) expression at the fetal-maternal interface from abortion-prone female mice that had previously received adoptive transfer of syngeneic DC. Syngeneic DC therapy induced an increase in the number of CD8 and gammadelta cells. Additionally, an upregulation of TGF-beta1 and PIBF expression could be detected after DC transfer. We suggest that DC therapy differentially upregulates a regulatory/protective population of cells at the fetal-maternal interface. It is reasonable to assure that this mechanism would be responsible for the lower abortion rate.
胎儿 - 胎盘单位是一种半同种异体移植，对妊娠的免疫识别以及母体免疫系统随后的反应是成功妊娠所必需的。树突状细胞（DC）具有生物学可塑性，赋予它们调节免疫和耐受的特殊特性。使用DC的治疗已被证明可减少DBA/2J与CBA/J交配雌性小鼠的流产；然而，其潜在机制仍然未知。在这里，我们评估了DC治疗是否会影响胎儿 - 母体界面免疫调节细胞群体的存在。为了验证这一假设，我们分析了先前接受同基因DC过继转移的易流产雌性小鼠胎儿 - 母体界面处具有妊娠保护作用的CD8、γδ细胞群体以及转化生长因子（TGF）-β1和孕酮诱导的封闭因子（PIBF）的表达。同基因DC治疗导致CD8和γδ细胞数量增加。此外，在DC转移后可检测到TGF-β1和PIBF表达上调。我们认为DC治疗可差异性地上调胎儿 - 母体界面处的调节性/保护性细胞群体。可以合理地确定这种机制是流产率降低的原因。
