Ji F, Luo X G, Lu L, Liu B, Yu S X
Mineral Nutrition Research Division, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100094, China.
Poult Sci. 2006 Nov;85(11):1947-52. doi: 10.1093/ps/85.11.1947.
Two experiments were conducted to investigate the effect of Mn source on Mn absorption by the intestine of broilers. In Experiment 1, the effect of Mn source, including MnSO(4), 2 Mn-amino acid chelates (Mn-Gly and Mn-Met) synthesized in our laboratory, 3 Mn-amino acid complexes with different complex strengths (Mn-Met E, Mn-AA A, and Mn-AA B), and 2 mixtures of MnSO(4) with Gly or Met, on Mn absorption was assessed with ligated loops of different small intestinal segments of broilers. In Experiment 2, the absorption of Mn from MnSO(4), Mn-AA A, and Mn-AA B was compared with intact broilers fed ad libitum. The criterion used for comparison was the Mn content of hepatic portal vein plasma. The absorption of Mn was higher (P < 0.0002) by ligated ileal loops than by duodenal and jejunal ones. Met supplementation increased (P < 0.03) the absorption of Mn as MnSO(4). The absorption of Mn as Mn-AA A and Mn-AA B with moderate and strong complex strengths, respectively, were higher (P < 0.05) than those of Mn as MnSO(4) and Mn-Met E with weak complex strength. On d 7 and 9 of Experiment 2, the Mn content of portal vein plasma was higher (P < 0.03) for Mn-AA B with strong complex strength than for MnSO(4). On d 9, Mn content in plasma was higher (P < 0.01) for Mn-AA B with strong complex strength than for Mn-AA A with a moderate one. The results from this study confirm that the ileum was the main site of Mn absorption for broilers, and Met was more effective in facilitating Mn absorption than Gly as a ligand. Organic Mn was more efficiently absorbed than inorganic Mn (MnSO(4)); the absorption of organic Mn with moderate and strong complex strengths was greater than that of the organic Mn, which was weak, and the absorption of organic Mn with strong complex strength was greater than that of the organic Mn with a moderate strength.
进行了两项试验以研究锰源对肉仔鸡肠道锰吸收的影响。在试验1中，采用肉仔鸡不同小肠段的结扎肠袢评估了锰源（包括硫酸锰、实验室合成的2种锰氨基酸螯合物（锰-甘氨酸和锰-蛋氨酸）、3种不同络合强度的锰氨基酸络合物（锰-蛋氨酸E、锰-氨基酸A和锰-氨基酸B）以及2种硫酸锰与甘氨酸或蛋氨酸的混合物）对锰吸收的影响。在试验2中，将硫酸锰、锰-氨基酸A和锰-氨基酸B的锰吸收情况与自由采食的完整肉仔鸡进行了比较。用于比较的标准是肝门静脉血浆中的锰含量。结扎回肠袢对锰的吸收高于十二指肠和空肠袢（P<0.0002）。添加蛋氨酸可提高硫酸锰形式的锰的吸收（P<0.03）。络合强度适中的锰-氨基酸A和络合强度较强的锰-氨基酸B的锰吸收分别高于络合强度较弱的硫酸锰和锰-蛋氨酸E（P<0.05）。在试验2的第7天和第9天，络合强度较强的锰-氨基酸B的门静脉血浆锰含量高于硫酸锰（P<0.03）。在第9天，络合强度较强的锰-氨基酸B的血浆锰含量高于络合强度适中的锰-氨基酸A（P<0.01）。本研究结果证实，回肠是肉仔鸡锰吸收的主要部位，作为配体，蛋氨酸促进锰吸收的效果比甘氨酸更有效。有机锰比无机锰（硫酸锰）吸收更高效；络合强度适中的和较强的有机锰的吸收大于弱络合强度的有机锰，且络合强度较强的有机锰的吸收大于络合强度适中的有机锰。
