美国榆（美洲榆）。

American Elm (Ulmus americana).

作者信息

Newhouse Andrew E, Schrodt Franziska, Maynard Charles A, Powell William A

机构信息

SUNY College of Environmental Science and Forestry, State Unviersity of New York, Syracuse, NY, USA.

出版信息

Methods Mol Biol. 2006;344:99-112. doi: 10.1385/1-59745-131-2:99.

DOI:10.1385/1-59745-131-2:99

PMID:17033055

Abstract

American elm (Ulmus americana) is a valuable and sentimental tree species that was decimated by Dutch elm disease in the mid-20th century. Therefore, any methods for modifying American elm or enhancing disease resistance are significant. This protocol describes transformation and tissue culture techniques used on American elm. Leaf pieces containing the midvein and petiole are used for explants. Agrobacterium tumefaciens strain EHA105 is used for transformation, with the binary vector pSE39, containing CaMV35S/nptII as a selectable marker, ACS2/ESF39A as a putative resistance enhancing gene, and CaMV35S/GUS as a reporter.

摘要

美国榆（Ulmus americana）是一种珍贵且具有情感价值的树种，在20世纪中叶因荷兰榆树病而大量死亡。因此，任何改良美国榆或增强其抗病性的方法都具有重要意义。本方案描述了用于美国榆的转化和组织培养技术。含有中脉和叶柄的叶片片段用作外植体。根癌农杆菌菌株EHA105用于转化，二元载体pSE39含有CaMV35S/nptII作为选择标记、ACS2/ESF39A作为假定的抗性增强基因以及CaMV35S/GUS作为报告基因。