Choi Yong Eui
Division of Forest Resources, College of Forest Sciences, Kangwon National University, Chunchon 200-701, Korea.
Methods Mol Biol. 2006;344:361-71. doi: 10.1385/1-59745-131-2:361.
Genetic transformation is an attractive way to improve Panax ginseng C. A. Meyer by introducing new genetic materials and altering metabolic pathways that regulating the production of secondary compounds. In P. ginseng, production of transgenic plants via Agrobacterium tumefaciens is performed via somatic embryogenesis using cotyledon or embryogenic callus as explants. This chapter introduces the protocol of A. tumefaciens mediated-genetic transformation in P. ginseng.
遗传转化是通过引入新的遗传物质和改变调节次生化合物产生的代谢途径来改良人参(Panax ginseng C. A. Meyer)的一种有吸引力的方法。在人参中,通过农杆菌介导利用子叶或胚性愈伤组织作为外植体,经体细胞胚胎发生来生产转基因植株。本章介绍人参农杆菌介导的遗传转化方案。