Luo J, Jankowski V, Henning L, Schlüter H, Zidek W, Jankowski J
Institute für Anatomie, Friedrich Schiller Universität Jena, Jena, Germany.
J Endocrinol Invest. 2006 Sep;29(8):688-93. doi: 10.1007/BF03344177.
Besides its role as a mechanical pump, the human heart serves as an endocrine organ, where known and as yet unknown hormones are produced. It is very likely that these hormones play an important role in cardiovascular regulation. In this study, a new endogenous vasoactive substance, coenzyme A glutathione disulfide (CoASSG), was isolated and identified in myocardial tissue. Human myocardial tissue was extracted with perchloric acid and fractionated by size exclusion-, displacement-, anion-exchange- and reversed-phase chromatography. In one fraction purified to homogeneity, CoASSG was identified by matrix assisted laser desorption/ionization (MALDI) mass-spectrometry, post-source decay MALDI-mass spectrometry and enzymatic structure analysis. Furthermore, CoASSG was also isolated from human cardiac specific granules. CoASSG has potent vasoconstrictive and proliferative effects. Therefore, CoASSG may affect myocardial function as an endocrine or autocrine substance after being released from myocardial specific granules.
除了作为一个机械泵的作用外，人类心脏还作为一个内分泌器官，产生已知和未知的激素。很可能这些激素在心血管调节中发挥重要作用。在本研究中，一种新的内源性血管活性物质，辅酶A谷胱甘肽二硫化物（CoASSG），在心肌组织中被分离和鉴定。用人心肌组织用高氯酸提取，并用尺寸排阻、置换、阴离子交换和反相色谱法进行分离。在一个纯化至同质的级分中，通过基质辅助激光解吸/电离（MALDI）质谱、源后衰变MALDI质谱和酶结构分析鉴定了CoASSG。此外，CoASSG也从人心脏特异性颗粒中分离出来。CoASSG具有强大的血管收缩和增殖作用。因此，CoASSG从心肌特异性颗粒释放后，可能作为一种内分泌或自分泌物质影响心肌功能。
