Ray Sanhita, Das Sanjoy K
Department of Pediatrics, Vanderbilt University Medical Center, Nashville, TN 37232, USA.
Biol Proced Online. 2006;8:69-76. doi: 10.1251/bpo120. Epub 2006 Jul 31.
Chromatin immunoprecipitation (ChIP) technique allows detection of proteins that bind to chromatin. While this technique has been applied extensively in cell-based studies, its tissue-based application remains poorly explored. We are specifically interested in examining estrogen-dependent transcriptional mechanism in respect of recruitment of estrogen receptor-alpha (ERalpha), a ligand-activated transcription factor, to uterine gene promoters in mice. Recent gene-array studies, utilizing ERalpha knock-out vs. wild-type mice, have revealed that estrogen regulates numerous uterine genes temporally and most importantly via ERalpha during the phase-II response, including three well characterized genes viz., lactoferrin (Ltf), progesterone receptor (Pgr) and cyclinD1 (Ccnd1). Here, utilizing systematic ChIP studies, we demonstrate endogenous recruitment of ERalpha to above uterine gene promoters following estradiol-17beta (E(2)) injection in mice.
染色质免疫沉淀（ChIP）技术可用于检测与染色质结合的蛋白质。尽管该技术已在基于细胞的研究中广泛应用，但其在基于组织的应用方面仍未得到充分探索。我们特别感兴趣的是研究雌激素依赖性转录机制，即配体激活的转录因子雌激素受体α（ERα）募集至小鼠子宫基因启动子的过程。最近利用ERα基因敲除小鼠与野生型小鼠进行的基因阵列研究表明，在II期反应期间，雌激素可在时间上调节众多子宫基因，并且最重要的是通过ERα进行调节，其中包括三个特征明确的基因，即乳铁蛋白（Ltf）、孕激素受体（Pgr）和细胞周期蛋白D1（Ccnd1）。在此，我们利用系统性的ChIP研究证明，在给小鼠注射17β-雌二醇（E₂）后，ERα可内源性募集至上述子宫基因启动子。
