Ohmori M, Endo T, Onaya T
Third Department of Internal Medicine, University of Yamanashi Medical School, Tamaho, Japan.
Biochem Biophys Res Commun. 1991 Jan 15;174(1):399-403. doi: 10.1016/0006-291x(91)90534-e.
We have synthesized four peptides (P2, P4, E3 and P1) corresponding to different segments of human thyrotropin (TSH) receptor. We have obtained antibodies by immunizing them to chickens, and antibodies are evaluated for their thyroid stimulating antibody (TSAb), thyroid stimulation blocking antibody (TSBAb) and TSH-binding inhibitor immunoglobulin (TBII) activities. None of the antibodies had TSAb activity. Antibodies against human TSH receptor specific region such as P2 and P4 (P2: No. 372-397, P4: No. 341-358) had TSBAb and TBII activities. Anti-E3 antibody (E3: the third putative extracellular loop, No. 649-661) had only TSBAb activity. Anti-P1 antibody (P1: high homology with pig LH/CG receptor, No. 398-417), however, had none. These results suggest that anti-TSH receptor antibodies to different antigenic epitopes show heterogeneity in their biological activities.
我们合成了四种与人促甲状腺激素（TSH）受体不同片段相对应的肽（P2、P4、E3和P1）。我们通过用它们免疫鸡获得了抗体，并对抗体的甲状腺刺激抗体（TSAb）、甲状腺刺激阻断抗体（TSBAb）和TSH结合抑制免疫球蛋白（TBII）活性进行了评估。没有一种抗体具有TSAb活性。针对人TSH受体特定区域（如P2和P4，P2：第372 - 397位，P4：第341 - 358位）的抗体具有TSBAb和TBII活性。抗E3抗体（E3：第三个假定的细胞外环，第649 - 661位）仅具有TSBAb活性。然而，抗P1抗体（P1：与猪促黄体激素/绒毛膜促性腺激素受体高度同源，第398 - 417位）则没有这些活性。这些结果表明，针对不同抗原表位的抗TSH受体抗体在其生物学活性上表现出异质性。
