Puglia Carmelo, Santagati Natale Alfredo, Bonina Francesco, Trombetta Domenico, Cristani Mariateresa, Speciale Antonio, Saija Antonella
Department of Pharmaceutical Sciences, School of Pharmacy, University of Catania, Catania, Italy.
J Pharm Pharmacol. 2006 Oct;58(10):1411-3. doi: 10.1211/jpp.58.10.0016.
Three oily extracts, obtained by acetone extraction from the entrails of different varieties of Mediterranean fishes, such as mackerel (Scomber scombrus), sardine (Sardina pilchardus) and horse mackerel (Trachurus mediterraneus), were characterized to determine their unsaturated fatty acid content. In an in-vitro model, their inhibitory effect was then evaluated against protein aggregation and their protective efficacy against heat-induced albumin denaturation assessed. The fish oil extracts tested in this study presented a significant amount of unsaturated fatty acids; in particular the extract obtained from the entrails of horse mackerel proved to have higher concentrations of DHA (docosahexaenoic acid) and oleic acid compared with the other two oils. The in-vitro study revealed an interesting protective effect of the oil extracts (particularly the horse mackerel extract) against heat-induced denaturation of albumin.
通过丙酮萃取从不同品种的地中海鱼类（如鲭鱼（Scomber scombrus）、沙丁鱼（Sardina pilchardus）和竹荚鱼（Trachurus mediterraneus））的内脏中获得了三种油性提取物，对其进行了表征以确定其不饱和脂肪酸含量。在体外模型中，随后评估了它们对蛋白质聚集的抑制作用以及对热诱导白蛋白变性的保护功效。本研究中测试的鱼油提取物含有大量不饱和脂肪酸；特别是从竹荚鱼内脏中获得的提取物，与其他两种油相比，DHA（二十二碳六烯酸）和油酸的浓度更高。体外研究揭示了油提取物（特别是竹荚鱼提取物）对热诱导白蛋白变性具有有趣的保护作用。
