Grover G J, Dzwonczyk S, Sleph P G
Department of Pharmacology, Squibb Institute for Medical Research, Princeton, New Jersey.
J Cardiovasc Pharmacol. 1990 Nov;16(5):783-9. doi: 10.1097/00005344-199011000-00014.
The effect of ruthenium red (inhibitor of mitochondrial calcium entry) on reperfusion contractile function and enzyme release was determined in isolated perfused rat hearts and compared with that of diltiazem. The hearts were made ischemic for 25 min and reperfused for 30 min. They were pretreated with 1-10 microM ruthenium red, 1 microM diltiazem, or vehicle. All concentrations of ruthenium red significantly improved reperfusion contractile function without affecting lactate dehydrogenase (LDH) release or contracture. Diltiazem significantly improved reperfusion function and reduced LDH release and contracture formation. Ruthenium red still improved function even when given only during reperfusion. Diltiazem increased reperfusion oxygen consumption and efficiency of oxygen utilization whereas ruthenium red improved efficiency without an increase in oxygen consumption. Diltiazem significantly increased reperfusion functional reserve in these hearts, although ruthenium red did not. Ruthenium red reduced coronary flow and contractile function in nonischemic myocardial tissue and the reduced function appeared to be secondary to the reduced coronary flow as well as to a direct negative inotropic effect. Thus, ruthenium red improved reperfusion contractile function and oxygen efficiency; this may be related to its ability to block mitochondrial calcium uptake.
在离体灌注大鼠心脏中测定钌红（线粒体钙内流抑制剂）对再灌注收缩功能和酶释放的影响，并与地尔硫䓬进行比较。心脏缺血25分钟，再灌注30分钟。用1 - 10微摩尔/升钌红、1微摩尔/升地尔硫䓬或赋形剂进行预处理。所有浓度的钌红均显著改善再灌注收缩功能，而不影响乳酸脱氢酶（LDH）释放或挛缩。地尔硫䓬显著改善再灌注功能，减少LDH释放和挛缩形成。即使仅在再灌注期间给予钌红，其仍能改善功能。地尔硫䓬增加再灌注氧消耗和氧利用效率，而钌红在不增加氧消耗的情况下提高效率。地尔硫䓬显著增加这些心脏的再灌注功能储备，而钌红则没有。钌红降低非缺血心肌组织的冠状动脉血流和收缩功能，且功能降低似乎继发于冠状动脉血流减少以及直接的负性肌力作用。因此，钌红改善再灌注收缩功能和氧效率；这可能与其阻断线粒体钙摄取的能力有关。
