Li Yanling, Meng Qingxiang
State Key Laboratory of Animal Nutrition, College of Animal Sciences & Technology, China Agricultural University, Beijing.
Arch Anim Nutr. 2006 Oct;60(5):402-11. doi: 10.1080/17450390600884401.
An in vitro study was conducted to determine the effect of different types of fibre supplemented with sunflower oil on ruminal fermentation and formation of conjugated linoleic acids (CLA) by mixed ruminal microorganisms. Cell wall components extracted from wheat straw (representing lignified fibre), soybean hulls (representing easily digestible fibre), and purified cellulose were used as substrates. Sunflower oil was supplemented at the same level for all three types of fibre. After 24 h of incubation, ruminal fermentation parameters (including 24 h gas production, pH value, concentration of ammonia nitrogen and volatile fatty acids) and the concentration of long chain fatty acids in the culture fluid were determined. Results showed that the type of fibre influenced ruminal fermentation traits and the biohydrogenation of unsaturated C18 fatty acids in vitro. Composition of LCFA and profile of CLA were altered by the fibre type. Compared to the digestible fibre and purified cellulose, lignified fibre significantly increased the production of cis-9, trans-11 CLA and total CLA (sum of cis-9, trans-11 CLA, trans-10, cis-12 CLA, trans-9, trans-11 CLA, and cis-9, cis-11 CLA) by ruminal microorganisms. It was concluded that ruminal fermentation and production of CLA can be affected by the type of dietary fibre.
进行了一项体外研究，以确定添加葵花籽油的不同类型纤维对瘤胃发酵以及混合瘤胃微生物共轭亚油酸（CLA）形成的影响。从小麦秸秆（代表木质化纤维）、大豆皮（代表易消化纤维）和纯化纤维素中提取的细胞壁成分用作底物。对所有三种类型的纤维添加相同水平的葵花籽油。培养24小时后，测定瘤胃发酵参数（包括24小时产气量、pH值、氨氮和挥发性脂肪酸浓度）以及培养液中长链脂肪酸的浓度。结果表明，纤维类型影响体外瘤胃发酵特性和不饱和C18脂肪酸的生物氢化。长链脂肪酸的组成和CLA的分布因纤维类型而异。与易消化纤维和纯化纤维素相比，木质化纤维显著增加瘤胃微生物产生的顺-9，反-11 CLA和总CLA（顺-9，反-11 CLA、反-10，顺-12 CLA、反-9，反-11 CLA和顺-9，顺-11 CLA的总和）。得出的结论是，膳食纤维的类型会影响瘤胃发酵和CLA的产生。
