Hwang M G, Katayama H, Ohgaki S
Department of Urban Engineering, University of Tokyo, 3-1-7 Hongo Bunkyo, Tokyo 133-8656, Japan.
Water Sci Technol. 2006;54(3):29-34. doi: 10.2166/wst.2006.444.
Pseudomonas aeruginosa, a gram-negative rod bacterium, is a causative agent of waterborne pneumonia and presents high tolerance against conventional disinfectants. The inorganic biocidal reagents, copper and silver, were applied to inactivate P. aeruginosa inoculated in a synthetic drinking water (SDW). Additionally, the relationship of the specific amount of accumulated copper and silver reagents (Cs) on P. aeruginosa with inactivation profile was elucidated in this study. Flow cytometry (FCM) following staining with SYTO 9 and PI was used for detection of bacterial viability and density. Individual copper and silver reagents, and their combination, exhibited excellent biocidal abilities even at the concentration of 0.05 mgCu/L and 0.005 mgAg/L. The critical amounts of accumulated disinfectant (Cs) were calculated at 2.82 x 10(-7) microgCu/cells and 5.13 x 10(-8) microgAg/cells; at an incubation of 70 h. Consequently, the role of disinfectant on the inactivation of P. aeruginosa and the assessment of biocidal ability of copper, silver, and their combination were successfully explained by evaluating the terms Cs and Cc.
铜绿假单胞菌是一种革兰氏阴性杆菌，是水源性肺炎的病原体，对传统消毒剂具有高度耐受性。无机杀生剂铜和银被用于灭活接种在合成饮用水（SDW）中的铜绿假单胞菌。此外，本研究阐明了铜绿假单胞菌上积累的铜和银试剂的特定量（Cs）与灭活曲线的关系。用SYTO 9和PI染色后的流式细胞术（FCM）用于检测细菌活力和密度。即使在0.05 mgCu/L和0.005 mgAg/L的浓度下，单独的铜和银试剂及其组合也表现出优异的杀菌能力。在70小时的孵育时间下，计算出积累消毒剂的临界量（Cs）为2.82×10（-7）μgCu/细胞和5.13×10（-8）μgAg/细胞。因此，通过评估Cs和Cc项，成功解释了消毒剂对铜绿假单胞菌灭活的作用以及铜、银及其组合的杀菌能力评估。
