Zhou Jian, Wang Lei, Du Jin-Min, Fan Yun-Liu
Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China.
Sheng Wu Gong Cheng Xue Bao. 2006 Sep;22(5):835-9.
Vitamin E (Tocopherols) is lipid-soluble antioxidants and essential for human health. Gamma-tocopherol methyltransferase (delta-TMT), one of the key enzymes in tocopherol biosynthetic pathway in plants, converts delta,sigma-tocopherols into alpha,beta-tocopherols. In this study, we isolated the 1552 bp promoter of Arabidopsis TMT gene. The promoter was fused with GUS reporter gene and this expression cassette was introduced into wild Arabidopsis thaliana by Agrobacterium-mediated transformation. GUS staining shows that GUS gene is expressed in leaves, stems and flowers, with the highest expression in young leaves, stamens and stem apices, while not observable in roots, seeds and siliques. The data indicate that gamma-TMT gene promoter is likely to be expressed preferentially in some of the tissues of Arabidopsis.
维生素E（生育酚）是脂溶性抗氧化剂，对人体健康至关重要。γ-生育酚甲基转移酶（δ-TMT）是植物生育酚生物合成途径中的关键酶之一，可将δ、σ-生育酚转化为α、β-生育酚。在本研究中，我们分离出了拟南芥TMT基因的1552 bp启动子。该启动子与GUS报告基因融合，通过农杆菌介导的转化将此表达盒导入野生型拟南芥中。GUS染色显示，GUS基因在叶片、茎和花中表达，在幼叶、雄蕊和茎尖中表达量最高，而在根、种子和角果中未观察到表达。数据表明，γ-TMT基因启动子可能在拟南芥的某些组织中优先表达。
