Montagne P
Laboratoire d'Immunologie, Faculté de Médecine de Nancy, Vandoeuvre.
Ann Biol Clin (Paris). 1990;48(7):459-64.
The main properties of solid/liquid interfaces are briefly considered before the weak chemical bonds involved in Ag-Ab interactions (hydrogen, electrostatic, Van Der Waals and hydrophobic bonds) are related in detail. The reversible nature of these non covalent bonds allows the discussion of the Ag-Ab reaction in the usual terms of chemical equilibrium the thermodynamic values associated with this equilibrium can be described. However, the complexity of the Ag-Ab reaction is strongly increased if Ag or Ab are bound to a solid phase. The modifications brought about by the solid phase are debated with the intention of understanding the physical and chemical parameters of the Ag-Ab reaction in solid phase immunoassays.
在详细阐述银-抗体相互作用中涉及的弱化学键（氢键、静电键、范德华键和疏水键）之前，先简要考虑固/液界面的主要特性。这些非共价键的可逆性使得可以用化学平衡的常用术语来讨论银-抗体反应，并描述与此平衡相关的热力学值。然而，如果银或抗体与固相结合，银-抗体反应的复杂性会大大增加。讨论了固相带来的修饰，目的是了解固相免疫分析中银-抗体反应的物理和化学参数。
