Sener A, Mourtada A, Blachier F, Malaisse W J
Laboratory of Experimental Medicine, Brussels Free University, Belgium.
Biochimie. 1990 Sep;72(9):685-8. doi: 10.1016/0300-9084(90)90052-i.
Cationic amino acids were recently found to stimulate amylase release from rat parotid cells. The possible relevance of their oxidative catabolism to such a secretory stimulation was investigated. D-Glucose, which was efficiently metabolized in parotid cells and which augmented O2 uptake above basal value, failed to affect basal or stimulated amylase release. L-Arginine, L-lysine and L-histidine failed to stimulate the oxidation of either exogenous D-[6-14C]glucose or endogenous nutrients in cells pre-labelled with [U-14C]palmitate or L-[U-14C]glutamine. The oxidation of L-[U-14C]arginine, L-[U-14C]ornithine, L-[U-14C]lysine and L-[U-14C]histidine, all tested at a 10 mM concentration, was much lower than that of D-[U-14C]glucose (5.6 mM). These findings argue against the view that the stimulation of amylase release by cationic amino acids would be related to their role as a source of energy in the parotid cells.
最近发现阳离子氨基酸可刺激大鼠腮腺细胞释放淀粉酶。研究了其氧化分解代谢与这种分泌刺激可能的相关性。D-葡萄糖在腮腺细胞中能有效代谢，并使耗氧量高于基础值，但它未能影响基础或刺激状态下的淀粉酶释放。L-精氨酸、L-赖氨酸和L-组氨酸未能刺激预先用[U-¹⁴C]棕榈酸酯或L-[U-¹⁴C]谷氨酰胺标记的细胞中外源D-[6-¹⁴C]葡萄糖或内源性营养物质的氧化。在10 mM浓度下测试的L-[U-¹⁴C]精氨酸、L-[U-¹⁴C]鸟氨酸、L-[U-¹⁴C]赖氨酸和L-[U-¹⁴C]组氨酸的氧化率远低于D-[U-¹⁴C]葡萄糖（5.6 mM）。这些发现与阳离子氨基酸刺激淀粉酶释放与其作为腮腺细胞能量来源的作用有关的观点相悖。
